Salivary Esterase Activity and Its Association with the Biodegradation of Dental Composites

Pseudocholinesterase (PCE) and cholesterol esterase (CE) can hydrolyze bisphenylglycidyl dimethacrylate (bisGMA) and triethylene glycol dimethacrylate (TEGDMA) monomers. This study will test the hypothesis that enzyme activities showing CE and PCE character are found in human saliva at levels suffic...

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Bibliographic Details
Published in:Journal of dental research 2004-01, Vol.83 (1), p.22-26
Main Authors: Finer, Y., Santerre, J.P.
Format: Article
Language:English
Subjects:
Analysis of Variance
Biodegradation, Environmental
Bisphenol A-Glycidyl Methacrylate - chemistry
Butyrylcholinesterase - metabolism
Cholinesterase Inhibitors - pharmacology
Chromatography, High Pressure Liquid
Composite Resins - chemistry
Dental Materials - chemistry
Enzyme Inhibitors - pharmacology
Humans
Hydrolysis
Mass Spectrometry
Phenylmethylsulfonyl Fluoride - pharmacology
Polyethylene Glycols - chemistry
Polymethacrylic Acids - chemistry
Saliva - enzymology
Species Specificity
Spectrophotometry, Ultraviolet
Sterol Esterase - antagonists & inhibitors
Sterol Esterase - metabolism
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Summary:Pseudocholinesterase (PCE) and cholesterol esterase (CE) can hydrolyze bisphenylglycidyl dimethacrylate (bisGMA) and triethylene glycol dimethacrylate (TEGDMA) monomers. This study will test the hypothesis that enzyme activities showing CE and PCE character are found in human saliva at levels sufficient to hydrolyze ester-containing composites important to restorative denstistry. The study also seeks to ask if the active sites of CE and PCE with respect to composite could be inhibited. Photo-polymerized model composite resin was incubated in PCE and CE solutions, in the presence and absence of a specific esterase inhibitor, phenylmethylsulfonyl fluoride (PMSF). Incubation solutions were analyzed for resin degradation products by high-performance liquid chromatography (HPLC), UV spectroscopy, and mass spectrometry. Saliva was found to contain both hydrolase activities at levels that could degrade composite resins. PMSF inhibited the composite degradation, indicating a material hydrolysis mechanism similar to the enzymes’ common function.
ISSN:0022-0345
1544-0591
DOI:10.1177/154405910408300105