Antifungal effect of phenolic extract of fermented rice bran with Rhizopus oryzae and its potential use in loaf bread shelf life extension

• Published in: Journal of the science of food and agriculture 2018-10, Vol.98 (13), p.5011-5018
• Main Authors: Denardi‐Souza, Taiana, Luz, Carlos, Mañes, Jordi, Badiale‐Furlong, Eliana, Meca, Giuseppe
• Format: Article
• Language: English
• Subjects: Antifungal activity; Antifungal Agents - metabolism; Antifungal Agents - pharmacology; Benzophenone; Bread; Bread - analysis; Bread - microbiology; Calcium; Ellagic acid; Fermentation; Fermented food; fermented rice bran; Flavonoids; Food Additives - metabolism; Food Additives - pharmacology; Food Contamination - analysis; Food Contamination - prevention & control; Food preservation; Food Storage; Fungi; Fungicides; Fusarium - drug effects; Fusarium - growth & development; Gallates; Life extension; Microbial Sensitivity Tests; Minimum inhibitory concentration; mycotoxigenic fungi; Organic acids; Organic chemistry; Oryza - chemistry; Oryza - microbiology; Penicillium - drug effects; Penicillium - growth & development; Phenolic compounds; Phenols; Phenols - metabolism; Phenols - pharmacology; Propionic acid; Rhizopus - metabolism; Rhizopus oryzae; Rice bran; Shelf life; shelf life extension
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.9035

BACKGROUND In this study the antifungal potential of a phenolic extract obtained from rice bran fermented with Rhizopus oryzae CECT 7560 and its application in the elaboration of bread was assessed. RESULTS Eighteen compounds with antifungal potential were identified by LC‐ESI‐qTOF‐MS in the extract: organic acids, gallates and gallotannins, flavonoids, ellagic acid and benzophenone derivatives. The extract was active against strains of Fusarium, Aspergillus and Penicillium, with minimum inhibitory concentration ranging from 390 to 3100 µg mL−1 and minimum fungicidal concentration variable from 780 to 6300 µg mL−1. The strains that were most sensitive to the phenolic extract were F. graminearum, F. culmorum, F. poae, P. roqueforti, P. expansum and A. niger. The phenolic extract added at 5 and 1 g kg−1 concentrations in the preparation of bread loaves contaminated with P. expansum produced a reduction of 0.6 and 0.7 log CFU g−1. The bread loaves treated with calcium propionate and 10 g kg−1 of the phenolic extract evidenced an improvement in their shelf lives of 3 days. CONCLUSION The phenolic extract assessed in this study could be considered as an alternative for inhibiting toxigenic fungi and as a substitute for synthetic compounds in food preservation. © 2018 Society of Chemical Industry