Environmental oxygen tension affects phenotype in cultured bone marrow-derived macrophages

This study tested the hypothesis that the unique phenotype of alveolar macrophages (AM) is maintained through adaptation to the relatively high oxygen partial pressure (Po2) of the lung, through modification of redox-sensitive transcription factors. BALB/c mouse bone marrowderived macrophages (BMC)...

Full description

Saved in:
Bibliographic Details
Published in:American journal of physiology. Lung cellular and molecular physiology 2004-02, Vol.30 (2), p.L354-L362
Main Authors: PFAU, Jean C, SCHNEIDER, Jordan C, ARCHER, Amy J, SENTISSI, Jami, LEYVA, Francisco J, CRAMTON, Jennifer
Format: Article
Language:English
Subjects:
Biological and medical sciences
Bone marrow
Cell culture
Cellular biology
Fundamental and applied biological sciences. Psychology
Genotype & phenotype
Lungs
Oxygen
Vertebrates: respiratory system
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study tested the hypothesis that the unique phenotype of alveolar macrophages (AM) is maintained through adaptation to the relatively high oxygen partial pressure (Po2) of the lung, through modification of redox-sensitive transcription factors. BALB/c mouse bone marrowderived macrophages (BMC) were differentiated under different Po2 and compared functionally to AM and peritoneal macrophages (PM). BMC differentiated in normoxia (Po2 140 Torr, BMChigh) were similar to AM in having low phagocytic and antigen presenting cell (APC) activities. However, BMC grown in low oxygen tension as found in other tissues (
ISSN:1040-0605
1522-1504