In-vitro and in-vivo evaluation of hemostatic potential of decellularized ECM hydrogels

•HECM hydrogels are biocompatible and do not contained toxic byproducts.•Increased platelet adhesion as ECM concentration in hydrogels increased.•Hemostatic properties increased as ECM concentration in hydrogels increased.•ECM proteins were attributed to good hemostatic properties of HECM hydrogels....

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Bibliographic Details
Published in:Materials letters 2018-12, Vol.232, p.130-133
Main Authors: Ventura, Reiza D., Padalhin, Andrew R., Lee, Byong Taek
Format: Article
Language:English
Subjects:
Biocompatibility
Biomedical materials
Bleeding
Cells
Crosslinking
Cytotoxicity
Extracellular matrix
Hemostat
Hemostatics
Hydrogel
Hydrogels
Materials science
Matrix
Microstructure
Platelets
Porcine dermal
Thrombin
Toxicity
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Summary:•HECM hydrogels are biocompatible and do not contained toxic byproducts.•Increased platelet adhesion as ECM concentration in hydrogels increased.•Hemostatic properties increased as ECM concentration in hydrogels increased.•ECM proteins were attributed to good hemostatic properties of HECM hydrogels.•HECM hydrogels decreased the bleeding time when applied in-vivo. Extracellular matrix (ECM) hydrogels with a porous microstructure and good swelling behavior were fabricated via lyophilization and cross-linked with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC). Platelet adhesion studies revealed that platelets easily adhered to hydrogels while coagulation studies showed rapid blood aggregate formation. In-vitro cytotoxicity studies showed ECM hydrogels are biocompatible. In-vivo studies using rat tail bleeding and rabbit arterial injury showed ECM hydrogels' ability in hemostasis. ECM hydrogels formed a physical barrier and activated platelets that resulted in rapid thrombin formation during in-vivo application. The present study revealed ECM hydrogels could be a potential biomaterial for hemostat application.
ISSN:0167-577X
1873-4979
DOI:10.1016/j.matlet.2018.08.013