Detection of microbial presence in the room, equipment, and cell culture media at temporary stem cell laboratory

Microbial existence is one of the standard indicators of room quality for stem cell culture laboratories by the Indonesian Health Ministry. During renovation of the Stem Cell Culture Laboratory in Surabaya, the laboratory technician designed a temporary laboratory for stem cell culture (SCC) on a re...

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Main Authors: Rahayu, Tri, Prasetya, Indra Adi Wira, Triwahyuni, Desi, Khiftiyah, Ana Mariatul, ‘Aini, Nurul, Bachruddin, Muhammad, Tsana, Izdihar, Marjayandari, Lisa, Istiqomah, Siti, Dewi, Selva Rosyta, Hanifah, Nabela Nur, Sholichah, Mar’atus, Fatimah, Ni’matuzahroh
Format: Conference Proceeding
Language:English
Subjects:
Carbon dioxide
Cell culture
Glass
Gloves
Health
Iodine
Laboratories
Media
Microorganisms
Molds
Pipettes
Safety cabinets
Stem cells
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Summary:Microbial existence is one of the standard indicators of room quality for stem cell culture laboratories by the Indonesian Health Ministry. During renovation of the Stem Cell Culture Laboratory in Surabaya, the laboratory technician designed a temporary laboratory for stem cell culture (SCC) on a research scale. The aim of this study was to examine sterility of the SCC processing room to determine whether it met the standard implemented by the Health Ministry of Indonesia. Microorganisms were isolated using direct plate, swap, and pour plate methods. Samples were collected from three points in the room. Equipment tested included CO2 incubator, biological safety cabinet, glass beaker, iodine cup, measuring pipettes, gloves, and Schott bottles. SCC media tested for microbes included adipose transport media, growth media, and media suspected of being contaminated by microbes. Descriptive analysis was based on microbial populations grown from each sample. Results showed that positive samples of bacterial infections included CO2 incubator, Biological Safety Cabinet, glass beakers, iodine cup, gloves, Schott bottle, and cell culture media suspected of microbial contamination. All samples were positive for infection by moulds and yeasts, except room, BSC, and growth media. This study indicated that the temporary laboratory did not meet stem cell culture room sterility standards set by the Health Ministry of Indonesia yet.
ISSN:0094-243X
1551-7616
DOI:10.1063/1.5061881