Role for Hydrogen Peroxide in Flow-Induced Dilation of Human Coronary Arterioles

ABSTRACT—Flow-induced dilation (FID) is dependent largely on hyperpolarization of vascular smooth muscle cells (VSMCs) in human coronary arterioles (HCA) from patients with coronary disease. Animal studies show that shear stress induces endothelial generation of hydrogen peroxide (H2O2), which is pr...

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Bibliographic Details
Published in:Circulation research 2003-02, Vol.92 (2), p.e31-e40
Main Authors: Miura, Hiroto, Bosnjak, John J, Ning, Gang, Saito, Takashi, Miura, Mamoru, Gutterman, David D
Format: Article
Language:English
Subjects:
Apamin - pharmacology
Arterioles - drug effects
Arterioles - physiology
Arterioles - ultrastructure
Catalase - pharmacology
Cerium
Coronary Vessels - drug effects
Coronary Vessels - physiology
Enzyme Inhibitors - pharmacology
Fluorescent Dyes
Glyburide - pharmacology
Humans
Hydrogen Peroxide - metabolism
Hydrogen Peroxide - pharmacology
In Vitro Techniques
Iron Chelating Agents - pharmacology
Membrane Potentials - drug effects
Membrane Potentials - physiology
Microscopy, Confocal
Microscopy, Electron
Microscopy, Video
Muscle, Smooth, Vascular - drug effects
Muscle, Smooth, Vascular - metabolism
Muscle, Smooth, Vascular - ultrastructure
Oxidants - metabolism
Oxidants - pharmacology
Papaverine - pharmacology
Potassium Channel Blockers - pharmacology
Stress, Mechanical
Vasodilation - drug effects
Vasodilation - physiology
Vasodilator Agents - pharmacology
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Summary:ABSTRACT—Flow-induced dilation (FID) is dependent largely on hyperpolarization of vascular smooth muscle cells (VSMCs) in human coronary arterioles (HCA) from patients with coronary disease. Animal studies show that shear stress induces endothelial generation of hydrogen peroxide (H2O2), which is proposed as an endothelium-derived hyperpolarizing factor (EDHF). We tested the hypothesis that H2O2 contributes to FID in HCA. Arterioles (135±7 μm, n=71) were dissected from human right atrial appendages at the time of cardiac surgery and cannulated with glass micropipettes. Changes in internal diameter and membrane potential of VSMCs to shear stress, H2O2, or to papaverine were recorded with videomicroscopy. In some vessels, endothelial H2O2 generation to shear stress was monitored directly using confocal microscopy with 2′,7′-dichlorofluorescin diacetate (DCFH) or using electron microscopy with cerium chloride. Catalase inhibited FID (%max dilation; 66±8 versus 25±7%;P
ISSN:0009-7330
1524-4571
DOI:10.1161/01.RES.0000054200.44505.AB