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AFLP-based genetic diversity assessment among wheat cultivars from the Pacific Northwest
Assessing genetic diversity among adapted, elite germplasm may expedite crop improvement. The objectives of this study were to (i) assess genetic diversity among a representative sample of spring and winter wheat (Triticum aestivum L.) cultivars adapted to dryland production in the Pacific Northwest...
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| Published in: | Crop science 1998-09, Vol.38 (5), p.1261-1271 |
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| container_end_page | 1271 |
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| container_title | Crop science |
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| creator | Barrett, B.A. (Washington State University, Pullman, WA.) Kidwell, K.K |
| description | Assessing genetic diversity among adapted, elite germplasm may expedite crop improvement. The objectives of this study were to (i) assess genetic diversity among a representative sample of spring and winter wheat (Triticum aestivum L.) cultivars adapted to dryland production in the Pacific Northwest by amplified fragment length polymorphisms (AFLPs), and (ii) compare genetic diversity estimates (GDEs) generated by methylation sensitive and methylation insensitive restriction enzymes for AFLP analyses. Fifty-tour cultivars and two diploid relatives were included in the study. Sixteen AFLP primer pairs detected 229 polymorphic bands. Mean GDEs (0.51 and 0.58, respectively) detected by PstI:MseI (methylation sensitive) and EcoRI:MseI (methylation insensitive) AFLP analyses were significantly different (P 0.0001), indicating greater diversity was detected for methylated sequences. Mean GDEs, based on data from both restriction enzyme combinations, were highest (0.58) for spring vs. winter type pairwise comparisons, intermediate (0.53) within winter type, and lowest (0.49) within spring type. Cluster analysis, ordination analysis, and the analysis of molecular variance suggested genetic diversity among cultivars was hierarchically arranged as cultivars nested within market class, and market classes nested within growth habit. AFLP analysis is an efficient technology for assessing genetic diversity among wheat cultivars |
| doi_str_mv | 10.2135/cropsci1998.0011183X003800050025x |
| format | article |
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The objectives of this study were to (i) assess genetic diversity among a representative sample of spring and winter wheat (Triticum aestivum L.) cultivars adapted to dryland production in the Pacific Northwest by amplified fragment length polymorphisms (AFLPs), and (ii) compare genetic diversity estimates (GDEs) generated by methylation sensitive and methylation insensitive restriction enzymes for AFLP analyses. Fifty-tour cultivars and two diploid relatives were included in the study. Sixteen AFLP primer pairs detected 229 polymorphic bands. Mean GDEs (0.51 and 0.58, respectively) detected by PstI:MseI (methylation sensitive) and EcoRI:MseI (methylation insensitive) AFLP analyses were significantly different (P 0.0001), indicating greater diversity was detected for methylated sequences. Mean GDEs, based on data from both restriction enzyme combinations, were highest (0.58) for spring vs. winter type pairwise comparisons, intermediate (0.53) within winter type, and lowest (0.49) within spring type. Cluster analysis, ordination analysis, and the analysis of molecular variance suggested genetic diversity among cultivars was hierarchically arranged as cultivars nested within market class, and market classes nested within growth habit. AFLP analysis is an efficient technology for assessing genetic diversity among wheat cultivars</description><identifier>ISSN: 0011-183X</identifier><identifier>EISSN: 1435-0653</identifier><identifier>DOI: 10.2135/cropsci1998.0011183X003800050025x</identifier><identifier>CODEN: CRPSAY</identifier><language>eng</language><publisher>Madison, WI: Crop Science Society of America</publisher><subject>Agronomy. Soil science and plant productions ; AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ; Arid zones ; ARIDOCULTURE ; Biological and medical sciences ; Botany ; CLADISTICS ; Crop improvement ; Cultivars ; CULTIVO EN TIERRAS ARIDAS ; DRY FARMING ; Enzymes ; Fundamental and applied biological sciences. Psychology ; Generalities. Genetics. Plant material ; Genetic aspects ; Genetic diversity ; GENETIC POLYMORPHISM ; Genetic polymorphisms ; Genetic research ; Genetic resources, diversity ; GENETIC VARIATION ; Genetics ; Genetics and breeding of economic plants ; GERMOPLASMA ; GERMPLASM ; HABITOS DE CRECIMIENTO ; HIVER ; INVIERNO ; Methods ; Methylation ; NUCLEOTIDE SEQUENCE ; PLANT HABIT ; Plant material ; POLIMORFISMO GENETICO ; POLYMORPHISME GENETIQUE ; PORT DE LA PLANTE ; PRIMAVERA ; PRINTEMPS ; SECUENCIA NUCLEOTIDICA ; SEQUENCE NUCLEOTIDIQUE ; SPRING ; SPRING AND WINTER HABIT ; TRITICUM AESTIVUM ; VARIACION GENETICA ; VARIATION GENETIQUE ; VARIEDADES ; VARIETE ; VARIETIES ; Wheat ; WINTER ; Winter wheat</subject><ispartof>Crop science, 1998-09, Vol.38 (5), p.1261-1271</ispartof><rights>Copyright © 1998 Crop Science Society of America</rights><rights>1998 INIST-CNRS</rights><rights>COPYRIGHT 1998 Crop Science Society of America</rights><rights>Copyright American Society of Agronomy Sep 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c520X-ab6f491b3820b5e4bdb1e69b716c90202c3ba4a44a5906cadfe8eff271aeca103</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2376827$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Barrett, B.A. (Washington State University, Pullman, WA.)</creatorcontrib><creatorcontrib>Kidwell, K.K</creatorcontrib><title>AFLP-based genetic diversity assessment among wheat cultivars from the Pacific Northwest</title><title>Crop science</title><description>Assessing genetic diversity among adapted, elite germplasm may expedite crop improvement. The objectives of this study were to (i) assess genetic diversity among a representative sample of spring and winter wheat (Triticum aestivum L.) cultivars adapted to dryland production in the Pacific Northwest by amplified fragment length polymorphisms (AFLPs), and (ii) compare genetic diversity estimates (GDEs) generated by methylation sensitive and methylation insensitive restriction enzymes for AFLP analyses. Fifty-tour cultivars and two diploid relatives were included in the study. Sixteen AFLP primer pairs detected 229 polymorphic bands. Mean GDEs (0.51 and 0.58, respectively) detected by PstI:MseI (methylation sensitive) and EcoRI:MseI (methylation insensitive) AFLP analyses were significantly different (P 0.0001), indicating greater diversity was detected for methylated sequences. Mean GDEs, based on data from both restriction enzyme combinations, were highest (0.58) for spring vs. winter type pairwise comparisons, intermediate (0.53) within winter type, and lowest (0.49) within spring type. Cluster analysis, ordination analysis, and the analysis of molecular variance suggested genetic diversity among cultivars was hierarchically arranged as cultivars nested within market class, and market classes nested within growth habit. AFLP analysis is an efficient technology for assessing genetic diversity among wheat cultivars</description><subject>Agronomy. Soil science and plant productions</subject><subject>AMPLIFIED FRAGMENT LENGTH POLYMORPHISM</subject><subject>Arid zones</subject><subject>ARIDOCULTURE</subject><subject>Biological and medical sciences</subject><subject>Botany</subject><subject>CLADISTICS</subject><subject>Crop improvement</subject><subject>Cultivars</subject><subject>CULTIVO EN TIERRAS ARIDAS</subject><subject>DRY FARMING</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Genetics. Plant material</subject><subject>Genetic aspects</subject><subject>Genetic diversity</subject><subject>GENETIC POLYMORPHISM</subject><subject>Genetic polymorphisms</subject><subject>Genetic research</subject><subject>Genetic resources, diversity</subject><subject>GENETIC VARIATION</subject><subject>Genetics</subject><subject>Genetics and breeding of economic plants</subject><subject>GERMOPLASMA</subject><subject>GERMPLASM</subject><subject>HABITOS DE CRECIMIENTO</subject><subject>HIVER</subject><subject>INVIERNO</subject><subject>Methods</subject><subject>Methylation</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>PLANT HABIT</subject><subject>Plant material</subject><subject>POLIMORFISMO GENETICO</subject><subject>POLYMORPHISME GENETIQUE</subject><subject>PORT DE LA PLANTE</subject><subject>PRIMAVERA</subject><subject>PRINTEMPS</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>SPRING</subject><subject>SPRING AND WINTER HABIT</subject><subject>TRITICUM AESTIVUM</subject><subject>VARIACION GENETICA</subject><subject>VARIATION GENETIQUE</subject><subject>VARIEDADES</subject><subject>VARIETE</subject><subject>VARIETIES</subject><subject>Wheat</subject><subject>WINTER</subject><subject>Winter wheat</subject><issn>0011-183X</issn><issn>1435-0653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqV0U2L2zAQBmBTWmi67U8omNJLod5qJMsfx2B224XQDU0XchNjeZRo8UcqOZvm31fGS-khl6KDQDyad4aJok_ArjkI-UW74eC1hbIsrhkDgEJsGRMFY0wyxuXvF9ECUiETlknxMlpMJpnQ6-iN94-B5WUuF9F2ebtaJzV6auId9TRaHTf2iZy34zlG78n7jvoxxm7od_FpTzjG-tiO9gmdj40bunjcU7xGbU34-31w4_5EfnwbvTLYenr3fF9FD7c3P6tvyer-6121XCVacrZNsM5MWkItCs5qSWnd1EBZWeeQ6ZJxxrWoMcU0RVmyTGNjqCBjeA5IGoGJq-jDXPfghl_HEKweh6PrQ6TiwGUBpYSAPs9ohy0p25thdKineR22Q0_GhuclBxAsEzLw5AIPp6HO6kt-OfuwFe8dGXVwtkN3VsDUtC71z7rUxXWFGh-f50CvsTUOe23930Jc5FnB88D0zE6hhfP_56hqU_Hqx_16U91N6hLahpT3c4rBQeHOhUYeNoGXgaUZiD-U971u</recordid><startdate>199809</startdate><enddate>199809</enddate><creator>Barrett, B.A. (Washington State University, Pullman, WA.)</creator><creator>Kidwell, K.K</creator><general>Crop Science Society of America</general><general>American Society of Agronomy</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>199809</creationdate><title>AFLP-based genetic diversity assessment among wheat cultivars from the Pacific Northwest</title><author>Barrett, B.A. (Washington State University, Pullman, WA.) ; Kidwell, K.K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c520X-ab6f491b3820b5e4bdb1e69b716c90202c3ba4a44a5906cadfe8eff271aeca103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>AMPLIFIED FRAGMENT LENGTH POLYMORPHISM</topic><topic>Arid zones</topic><topic>ARIDOCULTURE</topic><topic>Biological and medical sciences</topic><topic>Botany</topic><topic>CLADISTICS</topic><topic>Crop improvement</topic><topic>Cultivars</topic><topic>CULTIVO EN TIERRAS ARIDAS</topic><topic>DRY FARMING</topic><topic>Enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Genetics. Plant material</topic><topic>Genetic aspects</topic><topic>Genetic diversity</topic><topic>GENETIC POLYMORPHISM</topic><topic>Genetic polymorphisms</topic><topic>Genetic research</topic><topic>Genetic resources, diversity</topic><topic>GENETIC VARIATION</topic><topic>Genetics</topic><topic>Genetics and breeding of economic plants</topic><topic>GERMOPLASMA</topic><topic>GERMPLASM</topic><topic>HABITOS DE CRECIMIENTO</topic><topic>HIVER</topic><topic>INVIERNO</topic><topic>Methods</topic><topic>Methylation</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>PLANT HABIT</topic><topic>Plant material</topic><topic>POLIMORFISMO GENETICO</topic><topic>POLYMORPHISME GENETIQUE</topic><topic>PORT DE LA PLANTE</topic><topic>PRIMAVERA</topic><topic>PRINTEMPS</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>SPRING</topic><topic>SPRING AND WINTER HABIT</topic><topic>TRITICUM AESTIVUM</topic><topic>VARIACION GENETICA</topic><topic>VARIATION GENETIQUE</topic><topic>VARIEDADES</topic><topic>VARIETE</topic><topic>VARIETIES</topic><topic>Wheat</topic><topic>WINTER</topic><topic>Winter wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barrett, B.A. (Washington State University, Pullman, WA.)</creatorcontrib><creatorcontrib>Kidwell, K.K</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Crop science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barrett, B.A. (Washington State University, Pullman, WA.)</au><au>Kidwell, K.K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AFLP-based genetic diversity assessment among wheat cultivars from the Pacific Northwest</atitle><jtitle>Crop science</jtitle><date>1998-09</date><risdate>1998</risdate><volume>38</volume><issue>5</issue><spage>1261</spage><epage>1271</epage><pages>1261-1271</pages><issn>0011-183X</issn><eissn>1435-0653</eissn><coden>CRPSAY</coden><abstract>Assessing genetic diversity among adapted, elite germplasm may expedite crop improvement. The objectives of this study were to (i) assess genetic diversity among a representative sample of spring and winter wheat (Triticum aestivum L.) cultivars adapted to dryland production in the Pacific Northwest by amplified fragment length polymorphisms (AFLPs), and (ii) compare genetic diversity estimates (GDEs) generated by methylation sensitive and methylation insensitive restriction enzymes for AFLP analyses. Fifty-tour cultivars and two diploid relatives were included in the study. Sixteen AFLP primer pairs detected 229 polymorphic bands. Mean GDEs (0.51 and 0.58, respectively) detected by PstI:MseI (methylation sensitive) and EcoRI:MseI (methylation insensitive) AFLP analyses were significantly different (P 0.0001), indicating greater diversity was detected for methylated sequences. Mean GDEs, based on data from both restriction enzyme combinations, were highest (0.58) for spring vs. winter type pairwise comparisons, intermediate (0.53) within winter type, and lowest (0.49) within spring type. Cluster analysis, ordination analysis, and the analysis of molecular variance suggested genetic diversity among cultivars was hierarchically arranged as cultivars nested within market class, and market classes nested within growth habit. AFLP analysis is an efficient technology for assessing genetic diversity among wheat cultivars</abstract><cop>Madison, WI</cop><pub>Crop Science Society of America</pub><doi>10.2135/cropsci1998.0011183X003800050025x</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0011-183X |
| ispartof | Crop science, 1998-09, Vol.38 (5), p.1261-1271 |
| issn | 0011-183X 1435-0653 |
| language | eng |
| recordid | cdi_proquest_journals_212581951 |
| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Agronomy. Soil science and plant productions AMPLIFIED FRAGMENT LENGTH POLYMORPHISM Arid zones ARIDOCULTURE Biological and medical sciences Botany CLADISTICS Crop improvement Cultivars CULTIVO EN TIERRAS ARIDAS DRY FARMING Enzymes Fundamental and applied biological sciences. Psychology Generalities. Genetics. Plant material Genetic aspects Genetic diversity GENETIC POLYMORPHISM Genetic polymorphisms Genetic research Genetic resources, diversity GENETIC VARIATION Genetics Genetics and breeding of economic plants GERMOPLASMA GERMPLASM HABITOS DE CRECIMIENTO HIVER INVIERNO Methods Methylation NUCLEOTIDE SEQUENCE PLANT HABIT Plant material POLIMORFISMO GENETICO POLYMORPHISME GENETIQUE PORT DE LA PLANTE PRIMAVERA PRINTEMPS SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE SPRING SPRING AND WINTER HABIT TRITICUM AESTIVUM VARIACION GENETICA VARIATION GENETIQUE VARIEDADES VARIETE VARIETIES Wheat WINTER Winter wheat |
| title | AFLP-based genetic diversity assessment among wheat cultivars from the Pacific Northwest |
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