Single-base-resolution methylomes of Populus euphratica reveal the association between DNA methylation and salt stress

DNA methylation is an important biological form of epigenetic modification, playing key roles in plant development and environmental responses. In this study, we examined single-base resolution methylomes of Populus euphratica under control and salt stress conditions using high-throughput bisulfite...

Full description

Saved in:
Bibliographic Details
Published in:Tree genetics & genomes 2018-12, Vol.14 (6), p.1-11, Article 86
Main Authors: Su, Yutao, Bai, Xiaotao, Yang, Wenlu, Wang, Weiwei, Chen, Zeyuan, Ma, Jianchao, Ma, Tao
Format: Article
Language:English
Subjects:
Abiotic stress
Biomedical and Life Sciences
Biotechnology
Bisulfite
Deoxyribonucleic acid
DNA
DNA methylation
Forestry
Gene expression
Genome Biology
Leaves
Life Sciences
Original Article
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant tissues
Populus euphratica
Salts
Stresses
Transcription
Tree Biology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:DNA methylation is an important biological form of epigenetic modification, playing key roles in plant development and environmental responses. In this study, we examined single-base resolution methylomes of Populus euphratica under control and salt stress conditions using high-throughput bisulfite sequencing. Our data showed that the methylation levels of methylated cytosines in upstream 2 kb, downstream 2 kb, and repetitive sequences increased after salt treatment in leaves, but decreased in roots. We also found that heavy methylation in 100-bp upstream of the transcriptional start site repressed gene expression, while methylations within downstream 2 K and within the gene body were positively associated with gene expression. A total of 1893 and 1817 significant differentially methylated regions (DMRs) corresponding to 251 and 191 differentially methylated genes (DMGs) were identified in leaf and root tissues, respectively. These DMGs may play important roles in salt stress responses of P. euphratica through the changes of their DNA methylation levels. Overall, these findings provide valuable insights into our understanding of the interaction between gene expression and methylation of salt responses in poplars.
ISSN:1614-2942
1614-2950
DOI:10.1007/s11295-018-1298-1