Electron Microscopic Study of DNA Packing in [varphi]kz Bacteriophage (Pseudomonas Aeruginosa) Irradiated with Laser in the Presence of a Dye

DNA packing of bacteriophage phikz (Pseudomonas aeruginosa) was studied by electron microscopy after gentle destruction of the capsid with powerful laser impulse in aqueous virus suspension in the presence organic dye. Suspension of destroyed phage heads contained complexes of genomic DNA with endog...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2001-04, Vol.131 (4), p.356
Main Authors: Manykin, A A, Gabyshev, N N, Matsko, N B, Leonov, A G, Rudenko, A A, Chekhov, D I, Klimenko, S M
Format: Article
Language:English
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Summary:DNA packing of bacteriophage phikz (Pseudomonas aeruginosa) was studied by electron microscopy after gentle destruction of the capsid with powerful laser impulse in aqueous virus suspension in the presence organic dye. Suspension of destroyed phage heads contained complexes of genomic DNA with endogenous capsid proteins characterized by pronounced regular structure. The type of these structures corresponds to one the main model of phage DNA packing, the so-called coil packing.
ISSN:0007-4888
1573-8221