PS-280 Surface Film Fromation in Vitro By Infant And Therapeutic Surfactants: Role Of Surfactant Protein B

BackgroundEssential surfactant properties include transfer to gas-liquid interface, reduction of surface tension and film replenishment during respiratory cycles.ObjectiveTo compare component-specific film formation properties of infant and therapeutic surfactants.Design/methodsUsing a multiwell flu...

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Bibliographic Details
Published in:Archives of disease in childhood 2014-10, Vol.99 (Suppl 2), p.A213-A213
Main Authors: Danhaive, O, Chapin, C, Horneman, H, Cogo, PE, Ballard, PL
Format: Article
Language:English
Subjects:
Albumin
Anti-inflammatory agents
Budesonide
Fluorescence
Infants
Inflammation
Lipid composition
Palmitic acid
Phospholipids
Protein B
Respiratory distress syndrome
Surface tension
Surfactants
Young Children
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Summary:BackgroundEssential surfactant properties include transfer to gas-liquid interface, reduction of surface tension and film replenishment during respiratory cycles.ObjectiveTo compare component-specific film formation properties of infant and therapeutic surfactants.Design/methodsUsing a multiwell fluorescence assay, we compared maximal fluorescence (Max), time to reach Max (tMax) and phospholipid concentration for ½ maximal signal (½Max) for calfactant (CAL), poractant (POR), beractant (BER), colfosceryl palmitate (COL), with surfactant from immature infants with RDS. Dose-response studies were performed for addition of SP-B, albumin and budesonide.ResultsMax and ½Max values for CAL were higher/similar to those of rat surfactant. There were significant differences between CAL and other therapeutic surfactants for Max (CAL >COL >POR >BER) whereas ½Max were similar except for COL.In surfactant from 39 infant tracheal aspirates, ½Max was inversely correlated with SP-B content (p = 0.001). Addition of SP-B to samples with low endogenous content (
ISSN:0003-9888
1468-2044
DOI:10.1136/archdischild-2014-307384.580