Diffraction-limited axial scanning in thick biological tissue employing an aberration correcting adaptive lens

Diffraction-limited focusing deep into biological tissue is challenging due to spherical aberrations that lead to a broadening of the focal spot particularly in axial direction. While the diffraction-limit can be restored employing aberration correction with a deformable mirror or spatial light modu...

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Bibliographic Details
Published in:arXiv.org 2018-11
Main Authors: Philipp, Katrin, Lemke, Florian, Scholz, Stefan, Wallrabe, Ulrike, Wapler, Matthias C, Koukourakis, Nektarios, Czarske, Jürgen W
Format: Article
Language:English
Subjects:
Aberration
Deformation
Diffraction
Embryos
Fluorescence
Formability
Lenses
Spatial light modulators
Substructures
Thyroid gland
Zebrafish
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Summary:Diffraction-limited focusing deep into biological tissue is challenging due to spherical aberrations that lead to a broadening of the focal spot particularly in axial direction. While the diffraction-limit can be restored employing aberration correction with a deformable mirror or spatial light modulator, a bulky optical setup results due to the required beam-folding. We propose a bi actor adaptive lens, that enables axial scanning and at the same time correction of specimen induced spherical aberrations while offering a compact setup. Using the bi-actor lens in a confocal microscope, we show diffraction limited axial scanning up to \SI{340}{\micro m} deep inside a phantom specimen. Applying this technique for in-vivo measurements of zebrafish embryos with reporter gene-driven fluorescence in the thyroid gland reveals substructures of thyroid follicles.
ISSN:2331-8422
DOI:10.48550/arxiv.1811.11457