Transgenesis of schistosomes: approaches employing mobile genetic elements

Draft genome sequences for Schistosoma mansoni and Schistosoma japonicum are now available. However, the identity and importance of most schistosome genes have yet to be determined. Recently, progress has been made towards the genetic manipulation and transgenesis of schistosomes. Both loss-of-funct...

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Bibliographic Details
Published in:Parasitology 2008-02, Vol.135 (2), p.141-153
Main Authors: MANN, V. H., MORALES, M. E., KINES, K. J., BRINDLEY, P. J.
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified - genetics
Biological and medical sciences
DNA Transposable Elements - genetics
Drug resistance
electroporation
functional genomics
Fundamental and applied biological sciences. Psychology
gene manipulation
Gene therapy
General aspects
General aspects and techniques. Study of several systematic groups. Models
Genetic engineering
Genetic Engineering - methods
Genetics
Genomics
Interspersed Repetitive Sequences - genetics
Invertebrates
Mosquitoes
Nemathelminthia. Plathelmintha
Parasitology
promoter
reporter gene
Retroviridae - genetics
RNA interference
Schistosoma - genetics
schistosome
Transduction, Genetic - methods
transgenesis
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Summary:Draft genome sequences for Schistosoma mansoni and Schistosoma japonicum are now available. However, the identity and importance of most schistosome genes have yet to be determined. Recently, progress has been made towards the genetic manipulation and transgenesis of schistosomes. Both loss-of-function and gain-of-function approaches appear to be feasible in schistosomes based on findings described in the past 5 years. This review focuses on reports of schistosome transgenesis, specifically those dealing with the transformation of schistosomes with exogenous mobile genetic elements and/or their endogenous relatives for the genetic manipulation of schistosomes. Transgenesis mediated by mobile genetic elements offers a potentially tractable route to introduce foreign genes to schistosomes, a means to determine the importance of schistosome genes, including those that could be targeted in novel interventions and the potential to undertake large-scale forward genetics by insertional mutagenesis.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182007003824