Identification of a common recognition center for a photoactive non-steroidal antiinflammatory drug in serum albumins of different species

The non-steroidal anti-inflammatory drug (S)-carprofen (CPF) has been used as a photoactive probe to investigate the possible existence of a common recognition center in serum albumins (SAs) of different species. The methodology involves irradiation of the CPF/SA complexes, coupled with gel filtrati...

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Bibliographic Details
Published in:Organic chemistry frontiers an international journal of organic chemistry 2019-01, Vol.6 (1), p.99-109
Main Authors: Molins-Molina, Oscar, Lence, Emilio, Limones-Herrero, Daniel, González-Bello, Concepción, Miranda, Miguel A, M Consuelo Jiménez
Format: Article
Language:English
Subjects:
Albumins
Allosteric properties
Anti-inflammatory agents
Chromatography
Computer applications
Computer simulation
Coordination compounds
Covalence
Filtration
Gel chromatography
Gel filtration
Ibuprofen
Inflammation
Irradiation
Molecular docking
Molecular dynamics
Nonsteroidal anti-inflammatory drugs
Organic chemistry
Photolysis
Proteins
Radiation
Recognition
Trypsin
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Summary:The non-steroidal anti-inflammatory drug (S)-carprofen (CPF) has been used as a photoactive probe to investigate the possible existence of a common recognition center in serum albumins (SAs) of different species. The methodology involves irradiation of the CPF/SA complexes, coupled with gel filtration chromatography or proteomic analysis of the photolysates, docking and molecular dynamics simulations. Photolysis of CPF/SA complexes at λ = 320 nm, and gel filtration chromatography, revealed that the protein fraction still contained the drug fluorophore, in agreement with covalent attachment of the photogenerated radical intermediate CBZ· to SAs. After trypsin digestion and ESI-MS/MS, the incorporation of CBZ· was detected at several positions in the different albumins. Remarkably, modifications at the IB/IIIA interface were observed in all cases (Tyr452 in HSA, RbSA and RtSA and Tyr451 in BSA, PSA and SSA). The molecular basis of this common recognition, studied by docking and molecular dynamics simulation studies on the corresponding non-covalent complexes, corroborated the experimentally observed covalent modifications. Our computational studies also revealed that the previously reported displacement of CPF by (S)-ibuprofen, a site II specific drug, would be due to an allosteric effect in site II, rather than a direct molecular displacement, as expected.
ISSN:2052-4110
2052-4110
DOI:10.1039/c8qo01045e