Identification of Clematidis radix et Rhizoma and its adulterants by core haplotype based on the ITS sequences

To develop a method to identify Clematidis radix et Rhizoma using sequence similarity and sequence-specific genetic polymorphisms based on the ITS sequences. DNA was extracted from leaves of Clematis mandshurica Rupr and C. hexapetala using a DNA extraction kit. ITS sequences were amplified by PCR,...

Full description

Saved in:
Bibliographic Details
Published in:Genetics and molecular research 2018-04 (2), p.1
Main Authors: Zang, Yi-Mei, Gao, Ya, Liu, Ying, Liu, Chun-Sheng
Format: Article
Language:English
Subjects:
Deoxyribonucleic acid
DNA
Haplotypes
Nucleotide sequence
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To develop a method to identify Clematidis radix et Rhizoma using sequence similarity and sequence-specific genetic polymorphisms based on the ITS sequences. DNA was extracted from leaves of Clematis mandshurica Rupr and C. hexapetala using a DNA extraction kit. ITS sequences were amplified by PCR, and analyzed in Contig Express, DNAman, and MEGA 5.0. The core haplotype was determined, and similarities between the core and other haplotypes were calculated. In total, 138 ITS sequences of C. mandshurica were obtained with a length of 611 bp. The similarity threshold between C. mandshurica and counterfeit species was 99%. Using specific mutation sites, we could identify C. chinensis, C. hexapetala, and C. mandshurica rapidly and accurately. A new DNA-based method has been established to rapidly and accurately identify Clematidis radix et Rhizoma.
ISSN:1676-5680
DOI:10.4238/gmr16039905