A spermatogenesis-related gene expression profile in human spermatozoa and its potential clinical applications

Spermatogenesis is an essential stage in the human reproductive process. In a previously study aiming to determine which genes might be involved in spermatogenesis, we compared the gene expression profiles of adult and fetal testes by hybridizing cDNA probes prepared from adult and fetal testes to m...

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Bibliographic Details
Published in:Journal of molecular medicine (Berlin, Germany) Germany), 2004-05, Vol.82 (5), p.317-324
Main Authors: HUI WANG, ZUOMIN ZHOU, MIN XU, JIANMIN LI, JUNHUA XIAO, XU, Zhi-Yang, JIAHAO SHA
Format: Article
Language:English
Subjects:
A Kinase Anchor Proteins
Biological and medical sciences
Gene Expression - genetics
Gene Expression Profiling
General aspects
Glycoproteins - genetics
Heat-Shock Proteins - genetics
Humans
Infertility - etiology
Male
Medical sciences
Oligonucleotide Array Sequence Analysis
Protein Precursors - genetics
Sperm Motility - genetics
Spermatogenesis - genetics
Spermatozoa - metabolism
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Summary:Spermatogenesis is an essential stage in the human reproductive process. In a previously study aiming to determine which genes might be involved in spermatogenesis, we compared the gene expression profiles of adult and fetal testes by hybridizing cDNA probes prepared from adult and fetal testes to membranes dotted with gene clones derived from a commercial human testis library. We identified 266 differentially expressed genes that showed higher expression levels in adult testes, indicating their potential roles in spermatogenesis. In the present study, we applied the same cDNA microarray technique to the analysis of gene expression in the spermatozoa of normal fertile men and found 149 genes that were expressed at higher levels in adult testis. A further study of five sperm motility-related genes selected from this profile by real-time PCR revealed that there was significant difference in the expression levels of two genes ( TPX-1, testis-specific protein 1 and LDHC, lactate dehydrogenase C, transcript variant 1) between normal ( n=29) and motility impaired ( n=24) semen samples, indicating that these genes are involved in sperm function. Our results demonstrated that spermatogenesis-related gene profiling could help to assess sperm quality in humans, and further study of these genes will help us to elucidate the mechanisms involved in spermatogenesis and diseases relating to human infertility.
ISSN:0946-2716
1432-1440
DOI:10.1007/s00109-004-0526-3