Type III secretion machinery-deficient mutants of Ralstonia solanacearum lose their ability to colonize resulting in loss of pathogenicity

Abstract Ralstonia solanacearum OE1-1 (OE1-1) induced necrotic lesions in an infiltrated area of tobacco leaves 72 h after infiltration, and the leaves had wilted at 5 days. Here we report phenotypes of the OE1-1 mutant deleted hrpB or hrpY with respect to colonization and proliferation in infiltrat...

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Published in:Journal of general plant pathology : JGPP 2003-08, Vol.69 (4), p.250-257
Main Authors: Kanda, A. (Kochi Univ. (Japan)), Ohnishi, S, Tomiyama, H, Hasegawa, H, Yasukohchi, M, Kiba, A, Ohnishi, K, Okuno, T, Hikichi, Y
Format: Article
Language:English
Subjects:
COLONIZING ABILITY
GENE EXPRESSION
GENETIC DISORDERS
GENETIC ENGINEERING
Genotype & phenotype
Host plants
Infiltration
Leaves
Lesions
MUTANTS
Mutation
NICOTIANA TABACUM
PATHOGENICITY
Pathogens
Plant pathology
PSEUDOMONAS SOLANACEARUM
SECRETION
Tobacco
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Summary:Abstract Ralstonia solanacearum OE1-1 (OE1-1) induced necrotic lesions in an infiltrated area of tobacco leaves 72 h after infiltration, and the leaves had wilted at 5 days. Here we report phenotypes of the OE1-1 mutant deleted hrpB or hrpY with respect to colonization and proliferation in infiltrated tobacco leaves and the induction of host responses immediately after invasion. An hrpB-deleted mutant and an hrpY-deleted mutant grew similar to the parent strain, OE1-1, in vitro. When infiltrated into tobacco leaves, the mutants lost their ability to induce necrotic lesions and provoke the disease. Populations of the mutants in the infiltrated area were retained equally after infiltration, and the mutants were not detected in any other region. Transcripts of hsr203J and hin1, which are marker genes of plant-microbe interactions and were detected 8h after infiltration of OE1-1 in the infiltrated area of tobacco leaves, were not detected in the mutant-infiltrated tobacco leaves. These results suggest that the hrp mutants, which are deficient in type III secretion machinery, lose their ability to colonize and multiply in host plants immediately after invasion, resulting in a loss of their ability to induce host responses and the subsequent provocation of disease.
ISSN:1345-2630
1610-739X
DOI:10.1007/s10327-003-0041-3