Morphological studies on attachment of spores of Magnaporthe grisea to the leaf surface of rice [Oryza sativa]

Rice leaves were inoculated with spores of Magnaporthe grisea, and the number of fluorescence-labeled spores that attached to the leaf surface were counted before and after leaves were dipped and then stirred in water. Just 5% of the spores were retained on the leaf surface 1 h after inoculation; th...

Full description

Saved in:
Bibliographic Details
Published in:Journal of general plant pathology : JGPP 2004-02, Vol.70 (1), p.11-15
Main Authors: Koga, H. (Ishikawa Prefecture Coll. of Agriculture, Nonoichi (Japan)), Nakayachi, O
Format: Article
Language:English
Subjects:
FUNGAL MORPHOLOGY
FUNGAL SPORES
INFECTION
LEAVES
MAGNAPORTHE GRISEA
ORYZA SATIVA
Plant pathology
Rice
SURFACE AREA
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Rice leaves were inoculated with spores of Magnaporthe grisea, and the number of fluorescence-labeled spores that attached to the leaf surface were counted before and after leaves were dipped and then stirred in water. Just 5% of the spores were retained on the leaf surface 1 h after inoculation; the percentage retained then increased rapidly between 1.25 and 1.50 h, and most had attached by 2 h. Scanning electron microscopy revealed that most conidia were lying on a few wart-like protuberances 2-4 microm high. Spores became attached when the germ tubes were long enough to reach the leaf surface, at least 3 microm, by mucilaginous substances at the tip. Retained spores swayed when water was added under the cover glass from one side, indicating that the attachment was confined to the tips of germ tubes. Spores are attached to the rough leaf surface by mucilaginous substances--not at the tip of spore as reported on smooth artificial substrates but at the tip of the germ tubes. [PUBLICATION ABSTRACT]
ISSN:1345-2630
1610-739X
DOI:10.1007/s10327-003-0085-4