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The effect of sleep deprivation and disruption on DNA damage and health of doctors
Summary Observational studies have highlighted the detrimental health effects of shift work. The mechanisms through which acute sleep deprivation may lead to chronic disease have not been elucidated, but it is thought that increased DNA damage or decreased repair can lead to disease. The objective o...
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Published in: | Anaesthesia 2019-04, Vol.74 (4), p.434-440 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
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Online Access: | Get full text |
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Summary: | Summary
Observational studies have highlighted the detrimental health effects of shift work. The mechanisms through which acute sleep deprivation may lead to chronic disease have not been elucidated, but it is thought that increased DNA damage or decreased repair can lead to disease. The objective of this study was to examine the effects of acute sleep deprivation on DNA damage. This was a cross‐sectional observational study on 49 healthy, full‐time doctors. Baseline blood was sampled from each participant after three consecutive days of adequate sleep. Participants (n = 24) who were required to work overnight on‐site had additional blood sampled on a morning after acute sleep deprivation. DNA damage and expression of DNA repair genes were quantified. Information on health, working patterns and sleep diaries were collected. Independent t‐tests were used to compare differences between groups and standardised mean differences expressed as Cohen's d. Overnight on‐site call participants had lower baseline DNA repair gene expression and more DNA breaks than participants who did not work overnight (d = 1.47, p = 0.0001; and 1.48, p = 0.0001, respectively). In overnight on‐site call participants, after acute sleep deprivation, DNA repair gene expression was decreased (d = 0.90, p = 0.0001) and DNA breaks were increased (d = 0.87, p = 0.0018). Sleep deprivation in shift workers is associated with adverse health consequences. Increased DNA damage has been linked to the development of chronic disease. This study demonstrates that disrupted sleep is associated with DNA damage. Furthermore, larger prospective studies looking at relationships between DNA damage and chronic disease development are warranted, and methods to relieve, or repair, DNA damage linked to sleep deprivation should be investigated.
개요
여러 관찰 연구들에서 교대근무가 건강에 해로운 영향을 미치는 것으로 부각되었다. 아직 급성 수면결핍이 어떤 기전 을 통하여 만성 질환을 유도하는지 밝혀지지는 않았으나 DNA 손상의 증가 또는 복구의 감소가 원인이 될 수 있을 것 으로 생각된다. 이 연구의 목적은 급성 수면결핍이 DNA 손상 에 미치는 영향을 알아보고자 하는 것이다. 49명의 건강한 전 업 의사들을 대상으로 단면조사 관찰 연구를 수행하였다. 3일 간 충분한 수면을 취한 후 모든 대상자에게서 기저 혈액 샘플 을 채취하였다. 야간 당직근무를 하는 24명의 대상자들에서는 급성 수면결핍 다음날 아침 추가로 혈액 샘플을 채취하였다. DNA 손상 및 DNA 복구 유전자의 발현 정도를 정량적으로 분석하였다. 건강 상태, 근무 행태 및 수면일지에 대한 정보도 취득하였다. 독립표본 t‐검정을 통하여 군 간 차이를 비교하 였고 Cohen's d 로 표준화된 평균차를 표시하였다. 야간 당직 근무 의사는 당직근무를 하지 않는 의사들보다 기저에 DNA 복구 유전자 발현은 낮았고(d = 1.47, p = 0.0001), DNA 절단 은 많았다(d = 1.48, p = 0.0001). 당직근무를 하는 의사들에 서 급성 수면결핍 후에 DNA 복구 유전자 발현은 감소하였고 (d = 0.90, p = 0.0001), DNA 절단은 증가하였다(d = 0.87 |
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ISSN: | 0003-2409 1365-2044 |
DOI: | 10.1111/anae.14533 |