IGFs and IGF-Binding Proteins in the Synovial Fluid of Patients with Rheumatoid Arthritis and Osteoarthritis

The progressive damage of human articular cartilage is associated with loss of integrity of its extracellular matrix components. Their metabolism is under the control of cytokines produced locally. It is known that peptide growth factors stimulate chondrocytes to synthesize matrix components, and ot...

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Bibliographic Details
Published in:International journal of peptide research and therapeutics 2020-03, Vol.26 (1), p.271-280
Main Authors: Bączyk, Justyna, Gogiel, Tomasz, Wolańska, Małgorzata, Bruczko, Marta, Guszczyn, Tomasz, Popko, Janusz, Romanowicz, Lech
Format: Article
Language:English
Subjects:
Animal Anatomy
Bioavailability
Biochemistry
Biomedical and Life Sciences
Cartilage (articular)
Cartilage diseases
Chondrocytes
Cytokines
Enzyme-linked immunosorbent assay
Extracellular matrix
Growth factors
Histology
Insulin-like growth factor I
Interleukins
Inulin
Life Sciences
Metabolism
Molecular Medicine
Morphology
Osteoarthritis
Peptide growth factors
Pharmaceutical Sciences/Technology
Pharmacology/Toxicology
Polymer Sciences
Proteins
Rheumatoid arthritis
Synovial fluid
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Summary:The progressive damage of human articular cartilage is associated with loss of integrity of its extracellular matrix components. Their metabolism is under the control of cytokines produced locally. It is known that peptide growth factors stimulate chondrocytes to synthesize matrix components, and other cytokines, such as interleukins, promote their catabolism by stimulation of chondrocytes to the production of enzymes degrading components of cartilage. The aim of this study was evaluation the presence of inulin-like growth factors (IGFs) in synovial fluid and blood serum of patients with rheumatoid arthritis and osteoarthritis and their binding proteins and matrix metalloproteinases that regulate their bioavailability using Western Immunoblot, ELISA test and zymography technique. The results showed that both IGFs were present, first of all, in the form of high molecular complexes with their specific binding proteins. In this way those proteins prolonged growth factors’ half-life but suppressed their bioavailability for receptors and action on target cells. Low content of free IGF-I indicated limitation of its anabolic influence on cartilage metabolism of patients with both diseases.
ISSN:1573-3149
1573-3904
DOI:10.1007/s10989-019-09835-1