Characterization of Top Leader Elongation in Nordmann Fir (Abies nordmanniana)

Our understanding of the developmental changes that occur during top leader elongation in gymnosperms lags behind that in angiosperms. We developed a semiquantitative method for determining epidermal cell size, by measuring the Feret diameter after cell wall staining of stem epidermal peels. This me...

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Bibliographic Details
Published in:Journal of plant growth regulation 2019-12, Vol.38 (4), p.1354-1361
Main Authors: Martens, Helle Juel, Sørensen, Steen, Burow, Meike, Veierskov, Bjarke
Format: Article
Language:English
Subjects:
Abies nordmanniana
Agriculture
Angiosperms
Biomedical and Life Sciences
Cell number
Cell size
Cell walls
Cytokinins
Elongation
Fibonacci numbers
Geographical variations
Growth rate
Gymnosperms
Leadership
Life Sciences
Pine needles
Plant Anatomy/Development
Plant growth
Plant Physiology
Plant Sciences
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Summary:Our understanding of the developmental changes that occur during top leader elongation in gymnosperms lags behind that in angiosperms. We developed a semiquantitative method for determining epidermal cell size, by measuring the Feret diameter after cell wall staining of stem epidermal peels. This method allowed a large number of cells to be measured at various locations in the top leader of the Christmas tree Abies nordmanniana . Further, we have identified the growth rate of individual sections of the top leader, and the relationship between cell length and needle arrangement throughout the top leader. At bud break, all stem units begin to elongate simultaneously, but growth ceases from the base upwards during top leader elongation. Long top leaders were characterized by having up to three times as long cells at the base compared to short top leaders, whereas the cell lengths were similar in the apical region independent of the given plant growth capacity. In the basal sector, the level of auxin was much higher, whereas the levels of cytokinins were lower than in the apical sector, causing the auxin/cytokinin ratio to change from about 3 in the apical sector to more than 20 in the basal part. The Fibonacci number changed in the apical sector due to an increased cell number in the stem units and therefore longer distance between the needles. We conclude that the general growth pattern during top leader elongation in A. nordmanniana is similar to angiosperms but differs at the cellular level.
ISSN:0721-7595
1435-8107
DOI:10.1007/s00344-019-09938-5