Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory

The study aimed to determine the presence of mumps virus (MuV) in cerebrospinal fluid (CSF) specimens and to genetically characterise detected MuV strains. A real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the MuV F gene, and characterisation was performed by se...

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Bibliographic Details
Published in:Southern African journal of infectious diseases 2016-03, Vol.31 (1), p.29-31
Main Authors: Brauer, Marieke, Wolfaardt, Marianne, Webber, Lynne M, Taylor, Maureen B
Format: Article
Language:English
Subjects:
Aseptic meningitis
central nervous system
Cerebrospinal fluid
F gene
genotype
Mumps
Phylogeny
Polymerase chain reaction
Reverse transcription
Strains (organisms)
Vaccines
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Summary:The study aimed to determine the presence of mumps virus (MuV) in cerebrospinal fluid (CSF) specimens and to genetically characterise detected MuV strains. A real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the MuV F gene, and characterisation was performed by sequencing of the SH gene. Mumps virus was detected in 1.2% (3/260) of specimens. Phylogenetic analysis of one MuV strain revealed that it clustered with the Jeryl-Lynn and RIT4385 vaccine strains. As far as the authors could ascertain this is the first study to provide viral proof that these vaccine-like strains may be associated with aseptic meningitis.
ISSN:2312-0053
2313-1810
DOI:10.1080/23120053.2016.1118843