Increased protein carbonylation in leaves of Arabidopsis and soybean in response to elevated [CO₂]

• Published in: Photosynthesis research 2008-08, Vol.97 (2), p.155-166
• Main Authors: Qiu, Quan-Sheng, Huber, Joan L, Booker, Fitzgerald L, Jain, Vanita, Leakey, Andrew D. B, Fiscus, Edwin L, Yau, Peter M, Ort, Donald R, Huber, Steven C
• Format: Article
• Language: English
• Subjects: 2-Dimensional gel electrophoresis; Arabidopsis; Arabidopsis - drug effects; Arabidopsis - metabolism; Arabidopsis Proteins - metabolism; ascorbate peroxidase; Biochemistry; Biomedical and Life Sciences; C3 plants; Carbon dioxide; Carbon Dioxide - pharmacology; carbonylation; Glycine max; Glycine max - drug effects; Glycine max - metabolism; Life Sciences; ozone; Ozone - pharmacology; Photosynthesis; Plant Genetics and Genomics; Plant growth; Plant Leaves - drug effects; Plant Leaves - metabolism; Plant Physiology; plant proteins; plant response; Plant Sciences; Protein carbonylation; Protein Carbonylation - drug effects; Proteins; reactive oxygen species; Regular Paper; ribulose-bisphosphate carboxylase; Soybeans; two-dimensional polyacrylamide gel electrophoresis
• ISSN: 0166-8595; 1573-5079
• DOI: 10.1007/s11120-008-9310-5

While exposure of C₃ plants to elevated [CO₂] would be expected to reduce production of reactive oxygen species (ROS) in leaves because of reduced photorespiratory metabolism, results obtained in the present study suggest that exposure of plants to elevated [CO₂] can result in increased oxidative stress. First, in Arabidopsis and soybean, leaf protein carbonylation, a marker of oxidative stress, was often increased when plants were exposed to elevated [CO₂]. In soybean, increased carbonyl content was often associated with loss of leaf chlorophyll and reduced enhancement of leaf photosynthetic rate (Pn) by elevated [CO₂]. Second, two-dimensional (2-DE) difference gel electrophoresis (DIGE) analysis of proteins extracted from leaves of soybean plants grown at elevated [CO₂] or [O₃] revealed that both treatments altered the abundance of a similar subset of proteins, consistent with the idea that both conditions may involve an oxidative stress. The 2-DE analysis of leaf proteins was facilitated by a novel and simple procedure to remove ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from soluble soybean leaf extracts. Collectively, these findings add a new dimension to our understanding of global change biology and raise the possibility that oxidative signals can be an unexpected component of plant response to elevated [CO₂].