Transfer of a mutant plant glutamate 1-semialdehyde aminotransferase gene from the nuclear to the plastid genome confers gabaculine resistance in tobacco

New selection systems are required to extend plastid transformation to a more significant number of plant species. After demonstrating that a bacterial mutant glutamate 1-semialdehyde aminotransferase (GSA) gene is functional in tobacco plastids and confers resistance to the phytotoxin gabaculine, i...

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Published in:Plant cell, tissue and organ culture tissue and organ culture, 2019-05, Vol.137 (2), p.411-416
Main Authors: Bellucci, Michele, De Marchis, Francesca, Pompa, Andrea, Micheli, Maurizio, Gardi, Tiziano, Rosellini, Daniele
Format: Article
Language:English
Subjects:
Alfalfa
Biomedical and Life Sciences
Genetic transformation
Genomes
Gsa protein
Life Sciences
Phytotoxins
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Plant species
Plastids
Regeneration
Research Note
Spectinomycin
Tobacco
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Summary:New selection systems are required to extend plastid transformation to a more significant number of plant species. After demonstrating that a bacterial mutant glutamate 1-semialdehyde aminotransferase (GSA) gene is functional in tobacco plastids and confers resistance to the phytotoxin gabaculine, in this work, we transformed the tobacco plastome with a mutated GSA gene derived from alfalfa, along with the aadA selectable marker gene conferring spectinomycin resistance. Selection exploiting gabaculine was not effective to directly regenerate transplastomic events, but some events regenerated using conventional spectinomycin selection acquired gabaculine resistance during the second or third regeneration rounds with gabaculine selection. Gabaculine selection with our plant mutant GSA gene can be further investigated for plastid transformation of species that are not transformable with the aadA -spectinomycin selection system. Key Message Resistance to the phytotoxin gabaculine was obtained in tobacco by introducing a mutant plant gene in the plastid genome; it may be implemented as a tool in plastid genetic transformation.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-019-01566-x