Physicochemical, structural and biological evaluation of Cybersan (trigalactomargarate), a new glycolipid biosurfactant produced by a marine yeast, Cyberlindnera saturnus strain SBPN-27

• Published in: Process biochemistry (1991) 2019-05, Vol.80, p.171-180
• Main Authors: Senthil Balan, Shanmugasundaram, Ganesh Kumar, C., Jayalakshmi, Singaram
• Format: Article
• Language: English
• Subjects: Antimicrobial; Biocompatibility; Biomedical materials; Biosurfactant; Biosurfactants; Cyberlindnera saturnus; Cytotoxicity; Emulsification; Glycolipid; Growth inhibition; Mammals; Marine yeasts; Micelles; Natural products; Spectral analysis; Surface tension; Surfactants; Toxicity; Yeast; Yeasts
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2019.02.005

[Display omitted] •A potential non-hemolytic biosurfactant producing marine yeast Cyberlindnera saturnus SBPN 27.•New glycolipid structure identified as Gal-Gal-Gal-Heptadecanoic acid and named as Cybersan.•Cybersan exhibited excellent surface tension reduction, CMC and emulsification activity.•Cybersan revealed promising antibacterial activity with no cytotoxicity against mammalian cells.•Biological activities revealed its possible use as a mammalian safe antimicrobial drug. Recent attention on marine microbes has gained a renewed interest on natural product-based drug discovery with functional potential. Nevertheless, marine yeasts were less explored from a bioindustrial and biomedical perspective, especially on biosurfactants regardless no novel structures were identified till date. Considering these facts, this study screened biosurfactants from marine yeasts having broad spectrum industrial significance with the basic tensioactive properties, emulsification and non-hemolytic activities. Among the 136 strains isolated from three different stations of Tamil Nadu, India, Cyberlindnera saturnus SBPN-27 exhibited promising features. The biosurfactant from this marine yeast was purified and structurally characterized as Gal-Gal-Gal-Heptadecanoic acid (named as Cybersan) based on different spectral analysis. Further, Cybersan revealed surface tension of 28 mN m−1 at critical micelle concentration of 30 mg L−1 and stability over a broad pH and temperature conditions. Cybersan showed appreciable growth inhibition toward the clinical bacterial pathogens and revealed no considerable cytotoxicity against the mammalian 3T3 fibroblast cells suggesting its biocompatible nature. This is the first report on a new biosurfactant from a marine yeast having diverse functional properties and evidenced as promising candidate for mammalian safe antimicrobial therapy and other biomedical applications.