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Biocontrol of Botrytis cinerea by chitin-based cultures of Paenibacillus elgii HOA73
The chitinase-producing bacterium, Paenibacillus elgii HOA73, is a biocontrol agent that limits the damage caused to plants by microbial pathogens, insects, and nematodes. However, the mechanisms involved in the biocontrol of plant diseases by HOA73 have not been determined. The objective of this st...
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| Published in: | European journal of plant pathology 2019-09, Vol.155 (1), p.253-263 |
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| container_title | European journal of plant pathology |
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| creator | Kim, Young Cheol Hur, Jin Young Park, Seur Kee |
| description | The chitinase-producing bacterium,
Paenibacillus elgii
HOA73, is a biocontrol agent that limits the damage caused to plants by microbial pathogens, insects, and nematodes. However, the mechanisms involved in the biocontrol of plant diseases by HOA73 have not been determined. The objective of this study was to elucidate the role of extracellular chitinase obtained from isolate HOA73 in the control of the fungal pathogen
Botrytis cinerea
, the causative agent of gray mold in tomato. The HOA73 strain grew efficiently in a chitin-containing broth and produced chitin oligomers through chitinase activity; protease, lipase, and Fe-chelating siderophores were also secreted by the bacterium. Cultures containing intact bacteria inhibited
B. cinerea
conidia germination to a greater extent than did the bacterial cells alone or the cell-free culture supernatant. The antifungal activity increased with culture age and was heat-sensitive because of chitinase-mediated production of long-chain chitin oligomers. The biocontrol efficacy of undiluted bacterial cultures against gray mold in tomato was comparable to that of a standard fungicide. This study demonstrated that
P. elgii
HOA73 bacterial cultures grown on chitin-based minimal medium may be an effective formulation for the integrated control of gray mold. |
| doi_str_mv | 10.1007/s10658-019-01768-1 |
| format | article |
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Paenibacillus elgii
HOA73, is a biocontrol agent that limits the damage caused to plants by microbial pathogens, insects, and nematodes. However, the mechanisms involved in the biocontrol of plant diseases by HOA73 have not been determined. The objective of this study was to elucidate the role of extracellular chitinase obtained from isolate HOA73 in the control of the fungal pathogen
Botrytis cinerea
, the causative agent of gray mold in tomato. The HOA73 strain grew efficiently in a chitin-containing broth and produced chitin oligomers through chitinase activity; protease, lipase, and Fe-chelating siderophores were also secreted by the bacterium. Cultures containing intact bacteria inhibited
B. cinerea
conidia germination to a greater extent than did the bacterial cells alone or the cell-free culture supernatant. The antifungal activity increased with culture age and was heat-sensitive because of chitinase-mediated production of long-chain chitin oligomers. The biocontrol efficacy of undiluted bacterial cultures against gray mold in tomato was comparable to that of a standard fungicide. This study demonstrated that
P. elgii
HOA73 bacterial cultures grown on chitin-based minimal medium may be an effective formulation for the integrated control of gray mold.</description><identifier>ISSN: 0929-1873</identifier><identifier>EISSN: 1573-8469</identifier><identifier>DOI: 10.1007/s10658-019-01768-1</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agriculture ; Antifungal activity ; Bacteria ; Biological control ; Biomedical and Life Sciences ; Botrytis cinerea ; Cell culture ; Chelation ; Chitin ; Chitinase ; Conidia ; Ecology ; Fungicides ; Germination ; Grey mold ; Insects ; Integrated control ; Life Sciences ; Lipase ; Microorganisms ; Mold ; Nematodes ; Oligomers ; Paenibacillus ; Pathogens ; Plant diseases ; Plant Pathology ; Plant Sciences ; Plants (botany) ; Siderophores ; Tomatoes</subject><ispartof>European journal of plant pathology, 2019-09, Vol.155 (1), p.253-263</ispartof><rights>Koninklijke Nederlandse Planteziektenkundige Vereniging 2019</rights><rights>European Journal of Plant Pathology is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-1a444bca85f3ea155ef897083b7a9538424240377e98380b4782ab6a2a7de72d3</citedby><cites>FETCH-LOGICAL-c319t-1a444bca85f3ea155ef897083b7a9538424240377e98380b4782ab6a2a7de72d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Kim, Young Cheol</creatorcontrib><creatorcontrib>Hur, Jin Young</creatorcontrib><creatorcontrib>Park, Seur Kee</creatorcontrib><title>Biocontrol of Botrytis cinerea by chitin-based cultures of Paenibacillus elgii HOA73</title><title>European journal of plant pathology</title><addtitle>Eur J Plant Pathol</addtitle><description>The chitinase-producing bacterium,
Paenibacillus elgii
HOA73, is a biocontrol agent that limits the damage caused to plants by microbial pathogens, insects, and nematodes. However, the mechanisms involved in the biocontrol of plant diseases by HOA73 have not been determined. The objective of this study was to elucidate the role of extracellular chitinase obtained from isolate HOA73 in the control of the fungal pathogen
Botrytis cinerea
, the causative agent of gray mold in tomato. The HOA73 strain grew efficiently in a chitin-containing broth and produced chitin oligomers through chitinase activity; protease, lipase, and Fe-chelating siderophores were also secreted by the bacterium. Cultures containing intact bacteria inhibited
B. cinerea
conidia germination to a greater extent than did the bacterial cells alone or the cell-free culture supernatant. The antifungal activity increased with culture age and was heat-sensitive because of chitinase-mediated production of long-chain chitin oligomers. The biocontrol efficacy of undiluted bacterial cultures against gray mold in tomato was comparable to that of a standard fungicide. This study demonstrated that
P. elgii
HOA73 bacterial cultures grown on chitin-based minimal medium may be an effective formulation for the integrated control of gray mold.</description><subject>Agriculture</subject><subject>Antifungal activity</subject><subject>Bacteria</subject><subject>Biological control</subject><subject>Biomedical and Life Sciences</subject><subject>Botrytis cinerea</subject><subject>Cell culture</subject><subject>Chelation</subject><subject>Chitin</subject><subject>Chitinase</subject><subject>Conidia</subject><subject>Ecology</subject><subject>Fungicides</subject><subject>Germination</subject><subject>Grey mold</subject><subject>Insects</subject><subject>Integrated control</subject><subject>Life Sciences</subject><subject>Lipase</subject><subject>Microorganisms</subject><subject>Mold</subject><subject>Nematodes</subject><subject>Oligomers</subject><subject>Paenibacillus</subject><subject>Pathogens</subject><subject>Plant diseases</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Plants (botany)</subject><subject>Siderophores</subject><subject>Tomatoes</subject><issn>0929-1873</issn><issn>1573-8469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kM1KAzEURoMoWKsv4CrgOpqfmUmybIu2QqEu6jokaaamjJOaZBZ9e1NHcCeXy92c77twALgn-JFgzJ8SwU0tECayLG8EIhdgQmrOkKgaeQkmWFKJiODsGtykdMAlJCWdgO3cBxv6HEMHQwvnIcdT9gla37voNDQnaD989j0yOrkdtEOXh-jSGX7TrvdGW991Q4Ku23sPV5sZZ7fgqtVdcne_dwreX563ixVab5avi9kaWUZkRkRXVWWsFnXLnCZ17VohORbMcC1rJipaBjPOnRRMYFNxQbVpNNV85zjdsSl4GHuPMXwNLmV1CEPsy0tFKaNScCqbQtGRsjGkFF2rjtF_6nhSBKuzPTXaU8We-rGnSAmxMZQK3O9d_Kv-J_UNO69xOQ</recordid><startdate>20190901</startdate><enddate>20190901</enddate><creator>Kim, Young Cheol</creator><creator>Hur, Jin Young</creator><creator>Park, Seur Kee</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X2</scope><scope>88A</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20190901</creationdate><title>Biocontrol of Botrytis cinerea by chitin-based cultures of Paenibacillus elgii HOA73</title><author>Kim, Young Cheol ; Hur, Jin Young ; Park, Seur Kee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-1a444bca85f3ea155ef897083b7a9538424240377e98380b4782ab6a2a7de72d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Agriculture</topic><topic>Antifungal activity</topic><topic>Bacteria</topic><topic>Biological control</topic><topic>Biomedical and Life Sciences</topic><topic>Botrytis cinerea</topic><topic>Cell culture</topic><topic>Chelation</topic><topic>Chitin</topic><topic>Chitinase</topic><topic>Conidia</topic><topic>Ecology</topic><topic>Fungicides</topic><topic>Germination</topic><topic>Grey mold</topic><topic>Insects</topic><topic>Integrated control</topic><topic>Life Sciences</topic><topic>Lipase</topic><topic>Microorganisms</topic><topic>Mold</topic><topic>Nematodes</topic><topic>Oligomers</topic><topic>Paenibacillus</topic><topic>Pathogens</topic><topic>Plant diseases</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Plants (botany)</topic><topic>Siderophores</topic><topic>Tomatoes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Young Cheol</creatorcontrib><creatorcontrib>Hur, Jin Young</creatorcontrib><creatorcontrib>Park, Seur Kee</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>European journal of plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Young Cheol</au><au>Hur, Jin Young</au><au>Park, Seur Kee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biocontrol of Botrytis cinerea by chitin-based cultures of Paenibacillus elgii HOA73</atitle><jtitle>European journal of plant pathology</jtitle><stitle>Eur J Plant Pathol</stitle><date>2019-09-01</date><risdate>2019</risdate><volume>155</volume><issue>1</issue><spage>253</spage><epage>263</epage><pages>253-263</pages><issn>0929-1873</issn><eissn>1573-8469</eissn><abstract>The chitinase-producing bacterium,
Paenibacillus elgii
HOA73, is a biocontrol agent that limits the damage caused to plants by microbial pathogens, insects, and nematodes. However, the mechanisms involved in the biocontrol of plant diseases by HOA73 have not been determined. The objective of this study was to elucidate the role of extracellular chitinase obtained from isolate HOA73 in the control of the fungal pathogen
Botrytis cinerea
, the causative agent of gray mold in tomato. The HOA73 strain grew efficiently in a chitin-containing broth and produced chitin oligomers through chitinase activity; protease, lipase, and Fe-chelating siderophores were also secreted by the bacterium. Cultures containing intact bacteria inhibited
B. cinerea
conidia germination to a greater extent than did the bacterial cells alone or the cell-free culture supernatant. The antifungal activity increased with culture age and was heat-sensitive because of chitinase-mediated production of long-chain chitin oligomers. The biocontrol efficacy of undiluted bacterial cultures against gray mold in tomato was comparable to that of a standard fungicide. This study demonstrated that
P. elgii
HOA73 bacterial cultures grown on chitin-based minimal medium may be an effective formulation for the integrated control of gray mold.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10658-019-01768-1</doi><tpages>11</tpages></addata></record> |
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| subjects | Agriculture Antifungal activity Bacteria Biological control Biomedical and Life Sciences Botrytis cinerea Cell culture Chelation Chitin Chitinase Conidia Ecology Fungicides Germination Grey mold Insects Integrated control Life Sciences Lipase Microorganisms Mold Nematodes Oligomers Paenibacillus Pathogens Plant diseases Plant Pathology Plant Sciences Plants (botany) Siderophores Tomatoes |
| title | Biocontrol of Botrytis cinerea by chitin-based cultures of Paenibacillus elgii HOA73 |
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