A new glucose biosensor based on Nickel/KH550 nanocomposite deposited on the GCE: An electrochemical study

• Published in: Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2019-04, Vol.839, p.9-15
• Main Authors: Karami, Kazem, Bayat, Parvaneh, Allafchian, Ali Reza, Amiri, Razieh, Rezaei, Behzad, Laurents, Douglas V.
• Format: Article
• Language: English
• Subjects: Aluminum oxide; Aminopropyltriethoxysilane; Biosensors; Deionization; Electrodes; Electrons; Ethanol; Field emission microscopy; Fourier transforms; Glassy carbon; Glucose; Glucose sensor; Measurement methods; Microscopy; Nanocomposite; Nanocomposites; Nanoparticles; Nickel; Nickel nanoparticles; Oxidation; Scanning electron microscopy; Silver chloride; Synthesis; Transmission electron microscopy; Voltammetry; X-ray diffraction
• ISSN: 1572-6657; 1873-2569
• DOI: 10.1016/j.jelechem.2019.03.017

Quick, inexpensive and accurate methods are needed for glucose determination in many biochemical and medical labs. The purpose of this study is to develop and test a novel inexpensive electrode based on nickel/gamma-aminopropyltriethoxysilane nanoparticles for measuring glucose concentrations. First, the Ni nanoparticles (Ni NPs) were synthesized by a bottom-up approach. Then, the Ni NPs and gamma-Aminopropyltriethoxysilane (KH550) were mixed at 60°C under a nitrogen atmosphere to produce Ni/KH550 nanocomposite. Afterwards, the synthesized Ni/KH550 nanocomposites were characterized using different methods such as Fourier transform infrared (FT-IR), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD). Next, the surface of a glassy carbon electrode (GCE) was polished and washed with alumina powder and deionized water, respectively; and ultrasonicated in the H2O/ethanol solution. Then, the nanocomposite suspension was pipetted dropwise on the GCE surface and dried to GCE-Ni/KH550 production. The newly prepared electrode was characterized and applied to glucose detection utilizing electrochemical methods. The cyclic voltammetry measurements showed that the oxidation peak of glucose appeared at a potential of about 0.53V (vs. Ag/AgCl) on the surface of the modified GCE. Differential pulse voltammetry exhibited two wide linear dynamic ranges of 0.5–20 and 100–500μM glucose with a detection limit of 0.043μM. Finally, measurements of glucose in a real sample using our designed sensor indicated that GCE-Ni/KH550 is as good as those obtained with a high efficiency, commercial apparatus. [Display omitted] •The new Nickel/KH550 nanocomposite was used as modification factor of GCE.•The prepared electrode was utilized to glucose detection by electrochemical methods.•The results showed that GCE-Ni/KH550 can compete with commercial apparatus.