Development of a Universal Fluorescent Probe for Gram‐Positive Bacteria

The rapid and sensitive classification of bacteria is the first step of bacterial community research and the treatment of infection. Herein, a fluorescent probe BacGO is presented, which shows the best universal selectivity for Gram‐positive bacteria among known probes with a minimum staining proced...

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Bibliographic Details
Published in:Angewandte Chemie 2019-06, Vol.131 (25), p.8514-8519
Main Authors: Kwon, Haw‐Young, Liu, Xiao, Choi, Eun Gyeong, Lee, Jung Yeol, Choi, So‐Young, Kim, Jun‐Young, Wang, Lu, Park, Sung‐Jin, Kim, Beomsue, Lee, Yong‐An, Kim, Jong‐Jin, Kang, Nam Young, Chang, Young‐Tae
Format: Article
Language:English
Subjects:
Bacteria
Bacterial infections
Biologische Bildgebung
Borsäure
Chemistry
Communities
Fluorescent indicators
Fluoreszenzsonden
Gram-positive Bakterien
Infections
Keratitis
Selectivity
Sludge
Struktur-Aktivitäts-Beziehungen
Wastewater
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Summary:The rapid and sensitive classification of bacteria is the first step of bacterial community research and the treatment of infection. Herein, a fluorescent probe BacGO is presented, which shows the best universal selectivity for Gram‐positive bacteria among known probes with a minimum staining procedure for sample detection and enrichment of the live bacteria. BacGO could also be used to assess of the Gram status in the bacterial community from wastewater sludge. Furthermore, BacGO could sensitively and selectively detect a Gram‐positive bacterial infection, not only in vitro but also using an in vivo keratitis mouse model. BacGO provides an unprecedented research tool for the study of dynamic bacterial communities and for clinical application. BacGO, eine neuartige Gram‐positive Bakteriensonde, wurde aus einer Bibliothek fluoreszierender Moleküle mit Borsäuremotiv, das an Peptidoglykan in der Gram‐positiven Bakterienzellwand bindet, entwickelt. BacGO kann zur Identifizierung Gram‐positiver Bakterien in diversen, hochkomplexen Proben eingesetzt werden und ist eine attraktive Alternative zur Gram‐Färbung.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201902537