Nitrogen ions and nitrogen ion proportions impact the growth of apricot (Prunus armeniaca) shoot cultures

Nitrogen is a major driver of plant growth and the nitrogen source can be critical to good growth in vitro. A response surface methodology mixture-component design and a data mining algorithm were applied to nitrogen (N) nutrition for improving the micropropagation of Prunus armeniaca Lam. Data take...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2018-05, Vol.133 (2), p.263-273
Main Authors: Kovalchuk, Irina Y., Mukhitdinova, Zinat, Turdiyev, Timur, Madiyeva, Gulnara, Akin, Melekşen, Eyduran, Ecevit, Reed, Barbara M.
Format: Article
Language:English
Subjects:
Algorithms
Biomedical and Life Sciences
Calcium
Calcium ions
Callus
Data mining
Data processing
Disorders
In vitro methods and tests
Leaves
Life Sciences
Micropropagation
Necrosis
Nitrogen
Nitrogen ions
Nutrition
Original Article
Physiology
Plant diseases
Plant Genetics and Genomics
Plant growth
Plant Pathology
Plant Physiology
Plant Sciences
Prunus armeniaca
Regression analysis
Response surface methodology
Shoots
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Summary:Nitrogen is a major driver of plant growth and the nitrogen source can be critical to good growth in vitro. A response surface methodology mixture-component design and a data mining algorithm were applied to nitrogen (N) nutrition for improving the micropropagation of Prunus armeniaca Lam. Data taken on shoot cultures included a subjective quality rating, shoot number, shoot length, leaf characteristics and physiological disorders. Data were analyzed using the Classification and Regression Tree data mining algorithm. The best overall shoot quality as well as leaf color were on medium with NO 3 −  > 25 mM and NH 4 + /Ca + > 0.8. Improving shoot length to15 mm required 25  25 mM and NH 4 + /Ca 2+ ≤ 0.8, but there were 5–10 shoots at other NO 3 − concentrations regardless of NH 4 + /Ca 2+ proportion. Leaves increased in size with higher NO 3 − concentrations (> 55 mM). Physiological disorders were also influenced by the nitrogen components. Shoot tip necrosis was rarely present with NO 3 − > 45 mM. Callus production decreased somewhat with NH 4 + /Ca 2+ > 2.33. Suggested concentrations for an improved medium considering all of these growth characteristics would be 25 
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-018-1379-8