Binding preference of family 1 carbohydrate binding module on nanocrystalline cellulose and nanofibrillar cellulose films assessed by quartz crystal microbalance

• Published in: Cellulose (London) 2018-06, Vol.25 (6), p.3327-3337
• Main Authors: Zhang, Yu, Yang, Fang, Hu, Fanghui, Song, Junlong, Wu, Shufang, Jin, Yongcan
• Format: Article
• Language: English
• Subjects: Adsorption; Binding; Bioorganic Chemistry; Carbohydrates; Cellulose; Ceramics; Chemistry; Chemistry and Materials Science; Composites; Crystal structure; Crystallinity; Energy dissipation; Free energy; Gibbs free energy; Glass; Microbalances; Modules; Nanocrystals; Natural Materials; Organic Chemistry; Original Paper; Physical Chemistry; Polymer Sciences; Porosity; Quartz; Quartz crystals; Substrates; Sustainable Development
• ISSN: 0969-0239; 1572-882X
• DOI: 10.1007/s10570-018-1803-6

It is important to understand the interactions between the carbohydrate-binding module of fungal cellulases (CBM1) and the surface of cellulose because those interactions play an important role in the degradation of the crystalline regions of cellulose. In this investigation, interactions between isolated CBM1 and nanofibrillar and nanocrystalline cellulose (NFC and NCC, respectively) films were monitored with a quartz crystal microbalance with dissipation (QCM-D) in situ and in real time. The adsorption isotherms were employed to obtain the thermodynamic parameters of the interactions. Both Langmuir and Freundlich models appropriately describe the adsorption process of CBM1 on both types of films. Values of the Gibbs free energy associated with the adsorption of CBM1 on the NCC and NFC films were − 25.6 and − 23.7 kJ/mol, respectively. The results implied that the CBM1 binds spontaneously to both films but preferentially to NCC, with the differences attributed to variations in crystallinity and porosity of these substrates.