Competition for alfalfa nodulation under metal stress by the metal‐tolerant strain Ochrobactrum cytisi Azn6.2

• Published in: Annals of applied biology 2019-09, Vol.175 (2), p.184-192
• Main Authors: Navarro‐Torre, Salvadora, Rodríguez‐Llorente, Ignacio D., Doukkali, Bouchra, Caviedes, Miguel A., Pajuelo, Eloísa
• Format: Article
• Language: English
• Subjects: Alfalfa; Competition; Heavy metals; Kinetics; Legumes; Medicago sativa; Metal concentrations; multiresistance; Nitrogen enrichment; Nitrogen fixation; Nitrogenation; Nodulation; nodulation competitive assay; Nodules; Ochrobactrum; Sediment pollution; Soil pollution; Soils; Substrates; Symbiosis; α‐proteobacteria
• ISSN: 0003-4746; 1744-7348
• DOI: 10.1111/aab.12528

Legume plants, in association with rhizobia, are gaining increasing interest for heavy metal rhizoremediation. This symbiotic interaction combines the advantages of rhizoremediation and soil nitrogen enrichment. In metal polluted soils, Ochrobactrum cytisi can elicit non‐fixing nodules on legumes, including Medicago sativa. Nodulation kinetics was much slower when M. sativa plants were inoculated with O. cytisi Azn6.2 compared with the natural symbiont Ensifer meliloti 1021 and nodules were ineffective in nitrogen fixation. A competition experiment was performed using alfalfa grown on heavy metals, and co‐inoculated with equal amounts of the metal‐sensitive E. meliloti 1021 and the metal‐resistant O. cytisi Azn6.2. When plants were inoculated in non‐polluted substrates, all nodules were formed by E. meliloti 1021. Nevertheless, under increasing metal concentrations, the number of nodules occupied by O. cytisi grew. At the highest metal concentration, all nodules were elicited by O. cytisi, suggesting that the resistant species can take the place of the natural symbiont. This fact has important ecological and environmental implications when proposing legume–rhizobia symbioses for rhizoremediation and highlights the need of selecting highly resistant rhizobia in order to be competitive in polluted soils. A competition experiment was performed using alfalfa grown on heavy metals, and co‐inoculated with equal amounts of the metal‐sensitive Ensifer meliloti 1021 and the metal‐resistant Ochrobactrum cytisi Azn6.2. When plants were inoculated in non‐polluted substrates, all nodules were formed by E. meliloti 1021. Nevertheless, under increasing metal concentrations, the number of nodules occupied by O. cytisi grew. At the highest metal concentration, all nodules were elicited by O. cytisi, suggesting that the resistant species can take the place of the natural symbiont. These results highlight the need of selecting highly resistant rhizobia in order to be competitive in polluted soils.