Functions encoded by pyrrolnitrin biosynthetic genes from Pseudomonas fluorescens

Pyrrolnitrin is a secondary metabolite derived from tryptophan and has strong antifungal activity. Recently we described four genes, prnABCD, from Pseudomonas fluorescens that encode the biosynthesis of pyrrolnitrin. In the work presented here, we describe the function of each prn gene product. The...

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Bibliographic Details
Published in:Journal of Bacteriology 1998-04, Vol.180 (7), p.1939-1943
Main Authors: Kirner, S. (Universitat Hohenheim, Stuttgart.), Hammer, P.E, Hill, D.S, Altmann, A, Fischer, I, Weislo, L.J, Lanahan, M, Pee, K.H. van, Ligon, J.M
Format: Article
Language:English
Subjects:
ACIDE AMINE
AMINO ACIDS
AMINOACIDOS
ANTIBIOTICOS
ANTIBIOTICS
ANTIBIOTIQUE
Bacteriology
BIOSINTESIS
BIOSYNTHESE
BIOSYNTHESIS
Blotting, Western
COMPOSE ORGANOAZOTE
COMPOSE ORGANOCHLORE
COMPUESTO ORGANICO DEL CLORO
COMPUESTO ORGANICO DEL NITROGENO
ENZIMAS
ENZYME
ENZYMES
GENE
GENES
Genes, Bacterial
Genetics and Molecular Biology
METABOLISME DES PROTEINES
METABOLISMO PROTEICO
METABOLITE
METABOLITES
METABOLITOS
Microbiology
ORGANIC NITROGEN COMPOUNDS
ORGANOCHLORINE COMPOUNDS
Plasmids
PROTEIN METABOLISM
PSEUDOMONAS FLUORESCENS
Pseudomonas fluorescens - genetics
Pyrrolnitrin - biosynthesis
SECONDARY METABOLITES
STRUCTURAL GENES
TRIPTOFANO
TRYPTOPHAN
TRYPTOPHANE
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Description
Summary:Pyrrolnitrin is a secondary metabolite derived from tryptophan and has strong antifungal activity. Recently we described four genes, prnABCD, from Pseudomonas fluorescens that encode the biosynthesis of pyrrolnitrin. In the work presented here, we describe the function of each prn gene product. The four genes encode proteins identical in size and serology to proteins present in wild-type Pseudomonas fluorescens, but absent from a mutant from which the entire prn gene region had been deleted. The prnA gene product catalyzes the chlorination of L-tryptophan to form 7-chloro-L-tryptophan. The prnB gene product catalyzes a ring rearrangement and decarboxylation to convert 7-chloro-L-tryptophan to monodechloroaminopyrrolnitrin. The prnC gene product chlorinates monodechloroaminopyrrolnitrin at the 3 position to form aminopyrrolnitrin. The prnD gene product catalyzes the oxidation of the amino group of aminopyrrolnitrin to a nitro group to form pyrrolnitrin. The organization of the prn genes in the operon is identical to the order of the reactions in the biosynthetic pathway
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.180.7.1939-1943.1998