Injury and Recovery of Escherichia coli Cells in Phosphate-buffered Saline after High Hydrostatic Pressure Treatment

Escherichia coli cells suspended in phosphate-buffered saline (PBS) were treated by high hydrostatic pressure (HHP; 400 – 600 MPa) at 25 °C for 10 min and then stored at 5 – 25 °C. When treated at 600 MPa, the cells were inactivated lethally. Treatment at 400 and 500 MPa reduced viable cell counts b...

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Bibliographic Details
Published in:FOOD SCIENCE AND TECHNOLOGY RESEARCH 2019, Vol.25(3), pp.479-484
Main Authors: Morimatsu, Kazuya, Inaoka, Takashi, Nakaura, Yoshiko, Yamamoto, Kazutaka
Format: Article
Language:English
Subjects:
Bacteria
Buffers
Cannibalism
E coli
Escherichia coli
high hydrostatic pressure
Hydrostatic pressure
injury
Phosphates
Pressure
recovery
Storage
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Summary:Escherichia coli cells suspended in phosphate-buffered saline (PBS) were treated by high hydrostatic pressure (HHP; 400 – 600 MPa) at 25 °C for 10 min and then stored at 5 – 25 °C. When treated at 600 MPa, the cells were inactivated lethally. Treatment at 400 and 500 MPa reduced viable cell counts by approximately 7 and 8 log, respectively. Viable counts of cells treated at 400 or 500 MPa increased obviously during storage at 15 and 25 °C, whereas they did not increase at 5 and 10 °C. Meanwhile, healthy E. coli cells inoculated to a heat-killed dead cell suspension in PBS showed drastic growth during storage at 15 and 25 °C, but no growth at 5 and 10 °C. Therefore, the obvious increase in viable counts of HHP-treated cells might be attributed to their cannibalism of the dead cells.
ISSN:1344-6606
1881-3984
DOI:10.3136/fstr.25.479