Suitable carbon sources selection and ranking for biodiesel production by oleaginous Mucor circinelloides using multi-criteria analysis approach

• Published in: Fuel (Guildford) 2019-12, Vol.257, p.116117, Article 116117
• Main Authors: Kamoun, Omama, Muralitharan, Gangatharan, Belghith, Hafedh, Gargouri, Ali, Trigui-Lahiani, Hèla
• Format: Article
• Language: English
• Subjects: Agricultural wastes; Biodiesel; Biodiesel fuels; Biofuels; Carbon; Carbon sources; Chemical industry; Colorimetry; Cooking; Cooking oils; Crude oil; Diesel; Diet; Dietary supplements; Fatty acids; Fusarium; Identification methods; Industrial applications; Industrial by-products; Linoleic acid; Lipids; Microbial lipids; Microorganisms; Mucor circinelloides; Multiple criterion; Oil wastes; Oils & fats; Olive oil; Organic chemistry; Production costs; PROMETHEE-GAIA; Raw materials; Soybean oil; Soybeans; Substrates
• ISSN: 0016-2361; 1873-7153
• DOI: 10.1016/j.fuel.2019.116117

•Oleaginous fungi were screened by colorimetric and fluorimetric methods.•Accumulation of SCO in various stress conditions was investigated.•Ex novo lipid synthesis was strongly improved using industrial by-products.•Agro wastes influenced the degree of unsaturation of produced lipids.•PROMETHEE-GAIA algorithm used for the selection of potent substrates and strain. Crude oil is the most important raw material used in chemical industry and for energy supply. Microbial lipids are ideal for various industrial applications as an alternative to conventional fuel. However, the main factor controlling the total production costs is the choice of the growth substrates. For this reason, economically feasible substrate such as agro-industrial residues has been explored. In addition, the most suitable substrates for biodiesel production were selected by PROMETHEE-GAIA analysis on the basis of the fatty acid profile of the produced oils. Six strains were selected as the best lipid producer among twenty-three fungal strains screened by colorimetric methods. The strains identified belong to the genera Fusarium and Mucor. Mucor circinelloides was chosen for comparative evaluation of the accumulation performance with different carbon sources. The use of soap stock of refined soybean oil, waste cooking oils and soap stock of refined olive-pomace successfully resulted in improved lipid yield (4.07, 2.69 and 2.44 g/L) (ρ = 0.001; ρ > 0.05) and the lipid content at 46%, 51% and 44% (ρ = 0.049; ρ