Granulocyte colony-stimulating factor promotes adhesion of neutrophils

Granulocyte colony stimulating factor (G-CSF) is well known for its ability to drive the maturation and mobilization of neutrophils. G-CSF also appears to have the potential to activate functions of mature neutrophils, influencing recruitment at sites of inflammation and tissue injury. We investigat...

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Bibliographic Details
Published in:American Journal of Physiology: Cell Physiology 2003, Vol.53 (1), p.C103-C110
Main Authors: CHAKRABORTY, Arup, HENTZEN, Eric R, SEO, Scott M, SMITH, C. Wayne
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blood vessels and receptors
Cells
Fundamental and applied biological sciences. Psychology
Neurotransmitters
Vertebrates: cardiovascular system
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Summary:Granulocyte colony stimulating factor (G-CSF) is well known for its ability to drive the maturation and mobilization of neutrophils. G-CSF also appears to have the potential to activate functions of mature neutrophils, influencing recruitment at sites of inflammation and tissue injury. We investigated the ability of G-CSF to stimulate adhesion of isolated blood neutrophils. G-CSF induced significant adherence to intercellular adhesion molecule (ICAM)-1 that was both macrophage antigen-1 (Mac-1) and leukocyte function-associated antigen-1 dependent. The kinetics of G-CSF-stimulated adhesion to ICAM-1 peaked at 11 min without detectable surface upregulation of Mac-1. This was in marked contrast to chemokines, in which peak activation of adhesion is seen within 1 min of stimulation. In contrast to chemokine-induced adhesion, G-CSF stimulation was not inhibited by pertussis toxin. G-CSF also augmented the attachment of neutrophils to activated human umbilical vein endothelial cells (HUVEC) through specific effects on neutrophils, because HUVEC appear to lack functional G-CSF receptors.
ISSN:0363-6143
1522-1563