A critical look on CRISPR‐based genome editing in plants

Clustered regularly interspaced short palindromic repeats (CRISPR)‐based genome editing, derived from prokaryotic immunity system, is rapidly emerging as an alternative platform for introducing targeted alterations in genomes. The CRISPR‐based tools have been deployed for several other applications...

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Bibliographic Details
Published in:Journal of cellular physiology 2020-02, Vol.235 (2), p.666-682
Main Authors: Ahmad, Niaz, Rahman, Mehboob‐ur, Mukhtar, Zahid, Zafar, Yusuf, Zhang, Baohong
Format: Article
Language:English
Subjects:
Chromatin
CRISPR
CRISPR/Cas
crop improvement
Dependence
Editing
Epigenetics
Gene expression
Genetic diversity
Genetic engineering
Genetics
Genome editing
Genomes
Mutagenesis
Mutation
Phenotypes
Plant species
Regeneration
Reversion
risks
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Summary:Clustered regularly interspaced short palindromic repeats (CRISPR)‐based genome editing, derived from prokaryotic immunity system, is rapidly emerging as an alternative platform for introducing targeted alterations in genomes. The CRISPR‐based tools have been deployed for several other applications including gene expression studies, detection of mutation patterns in genomes, epigenetic regulation, chromatin imaging, etc. Unlike the traditional genetic engineering approaches, it is simple, cost‐effective, and highly specific in inducing genetic variations. Despite its popularity, the technology has limitations such as off‐targets, low mutagenesis efficiency, and its dependency on in‐vitro regeneration protocols for the recovery of stable plant lines. Several other issues such as persisted CRISPR activity in subsequent generations, the potential for transferring to its wild type population, the risk of reversion of edited version to its original phenotype particularly in cross‐pollinated plant species when released into the environment and the scarcity of validated targets have been overlooked. This article briefly highlights these undermined aspects, which may challenge the wider applications of this platform for improving crop genetics. Clustered regularly interspaced short palindromic repeats (CRISPR)‐based genome editing is rapidly emerging as an alternative platform for introducing targeted alterations in genomes. Despite its popularity, the technology has limitations such as off‐targets, low mutagenesis efficiency, and its dependency on in‐vitro regeneration protocols for the recovery of stable plant lines. This article highlights these undermined aspects briefly, which may challenge the wider application of this platform for improving crop genetics.
ISSN:0021-9541
1097-4652
DOI:10.1002/jcp.29052