Development of colchicine‐induced tetraploid St. Augustinegrass (Stenotaphrum secundatum) lines

St. Augustinegrass is well suited for lawns and commercial landscapes. While many genotypes are cross‐fertile, all cultivars are propagated vegetatively in sod production. To ensure varietal purity, development of sterile triploid hybrids by crossing tetraploid and diploid genotypes has been success...

Full description

Saved in:
Bibliographic Details
Published in:Plant breeding 2019-12, Vol.138 (6), p.958-966
Main Authors: Carbajal, Esdras M., Zuleta, M. Carolina, Swayzer, Luellen, Schwartz, Brian M., Chavarro, Maria Carolina, Ballen‐Taborda, A. Carolina, Milla‐Lewis, Susana R.
Format: Article
Language:English
Subjects:
artificial polyploids
chromosome doubling
Chromosomes
Colchicine
Cultivars
Flow cytometry
Genetic crosses
Genomes
Genotypes
Hybrids
Lawns
Plant growth
polyploidy breeding
Progeny
Purity
Seedlings
Seeds
Sod
St. Augustinegrass
Stenotaphrum secundatum
Turf
turfgrass
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:St. Augustinegrass is well suited for lawns and commercial landscapes. While many genotypes are cross‐fertile, all cultivars are propagated vegetatively in sod production. To ensure varietal purity, development of sterile triploid hybrids by crossing tetraploid and diploid genotypes has been successfully used in other warm‐season turfgrasses. Applying this model in St. Augustinegrass would be beneficial to sod producers and turf managers who require purity for certification and uniformity for performance, respectively. This study was conducted to develop colchicine‐induced tetraploid lines of St. Augustinegrass. Seeds of cultivar ‘Raleigh’ were treated with four colchicine concentrations at four exposure times. A non‐treated control was included among the treatments. Seedlings that germinated were screened for genome size changes using flow cytometry. Line DSA 13005 and two progeny lines derived through selfing, DSA 16001 and DSA 16016, were corroborated as tetraploids (2n = 4x = 36) through chromosome counts. These lines will be used in future breeding efforts to attempt development of sterile triploid cultivars of St. Augustinegrass.
ISSN:0179-9541
1439-0523
DOI:10.1111/pbr.12742