Novel Mixed Hydrotropic Solubalisation technique for Analytical method development and validation of Acyclovir in bulk and tablet formulation by Spectrophotometry

The absorbance was plotted against drug concentration and the linearity was thus calculated by the linear regression equation y= mx + c, where y represents the peak area and x represents drug concentration in g/mL. Assay: The average absorbance of the drug was computed from the spectrophotometer and...

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Bibliographic Details
Published in:Asian Journal of Pharmaceutical Analysis 2019-03, Vol.9 (1), p.25
Main Authors: Patil, S. S., Barhate, S. D., Patil, A. S., Patil, A. V., Patil, H. K., Salunke, P. A., Wagh, R. S.
Format: Article
Language:English
Subjects:
Antiviral drugs
Drug dosages
Herpes viruses
Methods
Pharmaceutical industry
Pharmaceutical sciences
Pharmaceuticals
Pharmacy
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Summary:The absorbance was plotted against drug concentration and the linearity was thus calculated by the linear regression equation y= mx + c, where y represents the peak area and x represents drug concentration in g/mL. Assay: The average absorbance of the drug was computed from the spectrophotometer and the amount of the drug present in the tablet dosage form was calculated by using the regression equation obtained for the pure drug. Validation parameters Result 1 UV detection wavelength (nm) 339 2 Linearity range (g/ml) 10-50 3 Standard regression equation y=0.012x+0.030 4 Correlation coefficient (R2) R2= 0.991 5 Precision (% RSD) Interday Intraday 0.8566 0.5300 6 Repeatability (n=6) 0.20 7 % Recovery (Accuracy, n=9) 80 103.24 100 105.37 120 106.79 8 LOD (g/ml) 1.21 9 LOQ (g/ml) 3.66 10 Assay (% Label claim) 101.48 CONCLUSION: [...]the solubility was increased as compared with distilled water.
ISSN:2231-5667
2231-5675
DOI:10.5958/2231-5675.2019.00007.3