PEG blocking of single pores arising on phase transitions in unmodified lipid bilayers

• Published in: Biophysics (Oxford) 2008-10, Vol.53 (5), p.390-395
• Main Authors: Antonov, V. F., Smirnova, E. Yu, Anosov, A. A., Norik, V. P., Nemchenko, O. Yu
• Format: Article
• Language: English
• Subjects: Biological and Medical Physics; Biophysics; Cell Biophysics; Lipids; Membranes; Molecular biology; Permeability; Physics; Physics and Astronomy
• ISSN: 0006-3509; 1555-6654
• DOI: 10.1134/S0006350908050126

Changes in ionic permeability of bilayer lipid membranes (BLM) from dipalmitoyl phosphatidylcholine at temperature of phase transition in 1 M LiCl solution in the presence of polyethyleneglycols (PEG) of various molecular masses are studied. The transition of ionic membrane channels from conducting to blocked nonconducting state using polymers makes it possible to calibrate lipid pores. It is shown that low-molecular weight glycerol and PEG with molecular weights of 300 and 600 decrease the amplitude of current fluctuations through the membrane, the decrease being proportional to the size of the polymer molecule incorporated. The addition of PEG with molecular masses of 1450, 2000, and 3350 decrease the current fluctuations to the basal noise level. The result is considered as a complete blockade of ion channel conductivity. In the presence of rather large polymers, such as PEG with molecular masses of 6000 and 20000, which are hardly incorporated in the pore, single current fluctuations occur again; however, their amplitudes are somewhat smaller than in the absence of PEG. It is assumed that a complete blockade of the conductivity of lipid ionic channels by PEG with molecular masses of 1450, 2000, and 3350 is due to dehydration of the pore gap and the conversion of the hydrophilic pore to a hydrophobic one.