Protective effects of Chrysin against memory impairment, cerebral hyperemia and oxidative stress after cerebral hypoperfusion and reperfusion in rats

• Published in: Metabolic brain disease 2020-02, Vol.35 (2), p.401-412
• Main Authors: Shooshtari, Maryam Khombi, Sarkaki, Alireza, Mansouri, Seyed Mohammad Taghi, Badavi, Mohammad, Khorsandi, Layasadat, Ghasemi Dehcheshmeh, Mohammad, Farbood, Yaghoob
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants; Biochemistry; Biomarkers; Biomedical and Life Sciences; Biomedicine; Blood flow; Brain; Cerebral blood flow; Cerebrovascular Circulation - drug effects; Cerebrovascular Circulation - physiology; Cerebrovascular Disorders - metabolism; Cerebrovascular Disorders - prevention & control; Cognition; Cognitive ability; Dose-Response Relationship, Drug; Flavonoids; Flavonoids - pharmacology; Flavonoids - therapeutic use; Hippocampus; Hyperemia; Hyperemia - metabolism; Hyperemia - prevention & control; Impairment; Ischemia; Male; Memory; Memory Disorders - metabolism; Memory Disorders - prevention & control; Metabolic Diseases; Morbidity; Neurology; Neuroprotection; Neuroprotective Agents - pharmacology; Neuroprotective Agents - therapeutic use; Neurosciences; Oncology; Original Article; Oxidative stress; Oxidative Stress - drug effects; Oxidative Stress - physiology; Parameters; Prostaglandin E2; Random Allocation; Rats; Rats, Wistar; Reperfusion; Rodents; Sensorimotor system; Surgery
• ISSN: 0885-7490; 1573-7365
• DOI: 10.1007/s11011-019-00527-9

Stroke is devastating and a leading cause of morbidity and mortality worldwide. Cerebral ischemia-reperfusion and its subsequent reactive hyperemia lead to neuronal damage in the hippocampus and cognitive decline. Chrysin (5, 7-dihydroxyflavone) is a well-known member of the flavonoid family with antioxidant and neuroprotective effects. Therefore, in the present study, the aim was to investigate whether chrysin will be able to recover the brain function caused by ischemia-reperfusion (I/R) in rats. Adult male Wistar rats (250–300 g) were randomly divided into five groups: and submitted to cerebral I/R or a sham surgery after three-weeks of pretreatment with chrysin (CH; 10, 30 and 100 mg/kg; P.O.) and/or normal saline containing %5 DMSO. Subsequently, sensorimotor scores, cognition, local cerebral blood flow, extracellular single unit, and histological parameters were evaluated following I/R. Hippocampus was used to evaluate biomarkers including: oxidative stress parameters and prostaglandin E2 (PGE2) using ELISA kits. Data showed that pretreatment with chrysin significantly improved sensorimotor signs, passive avoidance memory, and attenuated reactive hyperemia, and increased the average number of spikes/bin ( p