LC–MS untargeted approach showed that methyl jasmonate application on Vitis labrusca L. grapes increases phenolics at subtropical Brazilian regions

• Published in: Metabolomics 2020-02, Vol.16 (2), p.18, Article 18
• Main Authors: Moro, Laís, Da Ros, Alessio, da Mota, Renata Vieira, Purgatto, Eduardo, Mattivi, Fulvio, Arapitsas, Panagiotis
• Format: Article
• Language: English
• Subjects: Acetates - analysis; Acetates - metabolism; Biochemistry; Biomedical and Life Sciences; Biomedicine; Brazil; Cell Biology; Chromatography, High Pressure Liquid; Climate change; Cultivars; Cyclopentanes - analysis; Cyclopentanes - metabolism; Data analysis; Developmental Biology; Esters; Flavonoids; Glucosides; Life Sciences; Mass Spectrometry; Metabolites; Metabolomics; Methyl jasmonate; Methylation; Molecular Medicine; Original Article; Oxylipins - analysis; Oxylipins - metabolism; Phenolic compounds; Phenols; Phenols - analysis; Phenols - metabolism; Tartaric acid; Vitaceae; Viticulture; Vitis - chemistry; Vitis - metabolism; Vitis labrusca
• ISSN: 1573-3882; 1573-3890
• DOI: 10.1007/s11306-020-1641-z

Introduction Vitis labrusca L. grapes are largely cultivated in Brazil, but the tropical climate negatively affects the phenols content, especially anthocyanin. According to the projections of the incoming climatic changes, the climate of several viticulture zone might change to tropical. Therefore, researches are focusing on increasing grape phenols content; with methyl jasmonate application (MeJa) is considered a good alternative. Objectives The aim was to investigate with an untargeted approach the metabolic changes caused by the MeJa pre-harvest application on two Vitis labrusca L. cultivars grapes, both of them grown in two Brazilian regions. Methods Isabel Precoce and Concord grapes cultivated under subtropical climate, in the south and southeast of Brazil, received MeJa pre-harvest treatment. Grape metabolome was extracted and analyzed with a MS based metabolomics protocol by UPLC-HRMS-QTOF. Results Unsupervised data analysis revealed a clear separation between the two regions and the two cultivars, while supervised data analysis revealed biomarkers between the MeJa treatment group and the control group. Among the metabolites positively affected by MeJa were (a) flavonoids with a high degree of methylation at the B-ring (malvidin and peonidin derivatives and isorhamentin) for Isabel Precoce grapes; (b) glucosides of hydroxycinnamates, gallocatechin, epi gallocatechin and cis -piceid for Concord grapes; and (c) hydroxycinnamates esters with tartaric acid, and procyanidins for the Southeast region grapes. Conclusion These results suggest that MeJa can be used as elicitor to secondary metabolism in grapes grown even under subtropical climate, affecting phenolic biosynthesis.