An electrochemiluminescence sensor based on CdSe@CdS-functionalized MoS2 and a GOD-labeled DNA probe for the sensitive detection of Hg(ii)

In this study, a novel electrochemiluminescence (ECL) biosensor based on a CdSe@CdS quantum dot (QD)-functionalized MoS2-modified electrode was developed for the sensitive detection of mercury ions. Polycationic poly(diallyldimethylammonium chloride) (PDDA) and negatively charged QDs were adsorbed o...

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Bibliographic Details
Published in:Analytical methods 2020-01, Vol.12 (4), p.491-498
Main Authors: Zhang-Jin, He, Tian-Fang, Kang, Li-Ping, Lu, Shui-Yuan, Cheng
Format: Article
Language:English
Subjects:
Absorption spectroscopy
Biosensors
Cadmium selenides
Cadmium sulfide
Composite materials
Deoxyribonucleic acid
Dissolved oxygen
DNA
Electrochemiluminescence
Electrodes
Fluorescence
Gene sequencing
Glucose oxidase
Gold
Hydrogen peroxide
Mercury
Mercury (metal)
Mercury compounds
Molybdenum disulfide
Nanoparticles
Nucleotide sequence
Quantum dots
Thymine
Transmission electron microscopy
Water analysis
Water sampling
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Summary:In this study, a novel electrochemiluminescence (ECL) biosensor based on a CdSe@CdS quantum dot (QD)-functionalized MoS2-modified electrode was developed for the sensitive detection of mercury ions. Polycationic poly(diallyldimethylammonium chloride) (PDDA) and negatively charged QDs were adsorbed on the surface of MoS2, in sequence, to form the QD–PDDA–MoS2 composites, which were employed as matrices for immobilizing thymine-rich DNA sequences (DNA1). The gold nanoparticles (AuNPs) were combined with another type of thymine-rich DNA sequences (DNA2) and glucose oxidase (GOD) to prepare the GOD–AuNP–DNA2 conjugates. The composites were characterized via UV-visible absorption spectroscopy, fluorescence and transmission electron microscopy. A thymine–Hg2+–thymine complex was formed through Hg2+ mismatching with DNA1 and DNA2. GOD was, therefore, modified onto the electrode surface. GOD catalyzed the reduction of dissolved oxygen by glucose to produce hydrogen peroxide, which was a co-reactant of the QDs. The electrochemiluminescence signal of the biosensor increased linearly with the increase in the Hg2+ concentration over the range from 1.0 × 10−12 to 1.0 × 10−6 M with a detection limit of 1 × 10−13 M. The reproducibility, stability and specificity of the biosensor was also studied. The biosensor was used to determine Hg2+ in a real water sample and satisfactory results were obtained.
ISSN:1759-9660
1759-9679
DOI:10.1039/c9ay02524c