Comparison of a dipstick enzyme-linked immunosorbent assay with commercial assays for detection of Japanese encephalitis virus-specific IgM antibodies

Background: Japanese encephalitis (JE) is a major public health concern in Asia including India. Objectives: To evaluate an in-house developed dipstick enzyme-linked immunosorbent assay (ELISA) test vis-à-vis two commercial kits for detection of JE virus-specific IgM antibodies. Setting and Design:...

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Bibliographic Details
Published in:Journal of postgraduate medicine (Bombay) 2008-07, Vol.54 (3), p.181
Main Authors: Shrivastva, A, Tripathi, NK, Parida, M, Dash, PK, Jana, AM, Lakshmana, Rao PV
Format: Article
Language:English
Subjects:
Antibody, diagnostic test, dipstick enzyme-linked immunosorbent assay, Japanese encephalitis, virus
Care and treatment
Cells
Developing countries
Diagnosis
Encephalitis
Enzyme-linked immunosorbent assay
Identification and classification
Immunoglobulin M
Infections
Japanese encephalitis
LDCs
Methods
Monoclonal antibodies
Patients
Physiological aspects
Polyethylene glycol
Signal to noise ratio
Viruses
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Summary:Background: Japanese encephalitis (JE) is a major public health concern in Asia including India. Objectives: To evaluate an in-house developed dipstick enzyme-linked immunosorbent assay (ELISA) test vis-à-vis two commercial kits for detection of JE virus-specific IgM antibodies. Setting and Design: Comparative study carried out in Research and Development centre. Materials and Methods: A total of 136 specimens comprising 84 serum and 52 CSF samples were tested by in-house dipstick ELISA, Pan-Bio IgM capture ELISA (Pan-Bio, Australia) and JEV CheX IgM capture ELISA (XCyton, India). Results: The overall agreement among all three tests was found to be 92% with both serum and cerebrospinal fluid (CSF) samples. The sensitivity of the dipstick ELISA was found to be 91% with serum and 89% with CSF samples respectively. The specificity of the dipstick ELISA with reference to both commercial assays was found to be 100% in serum and CSF samples in this study. Conclusions: The in-house dipstick ELISA with its comparable sensitivity and specificity can be used as a promising test in field conditions since it is simple, rapid and requires no specialized equipment.
ISSN:0022-3859
0972-2823
DOI:10.4103/0022-3859.40959