Differential partitioning of thiols and glucosinolates between shoot and root in Chinese cabbage upon excess zinc exposure

Zinc (Zn) is one of the important elements of plant growth, however, at elevated level it is toxic. Exposure of Chinese cabbage to elevated Zn2+ concentrations (5 and 10 μM ZnCl2) resulted in enhancement of total sulfur and organic sulfur concentration. Transcript level of APS reductase (APR) as a k...

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Bibliographic Details
Published in:Journal of plant physiology 2020-01, Vol.244, p.153088, Article 153088
Main Authors: Aghajanzadeh, Tahereh A., Prajapati, Dharmendra H., Burow, Meike
Format: Article
Language:English
Subjects:
ATP sulfurylase
Biosynthesis
Brassica - metabolism
Brassica oleracea
Chinese cabbage
Cysteine
Enzymes
Exposure
Gene expression
Glucosinolates
Glucosinolates - metabolism
Plant growth
Plant Proteins - metabolism
Plant Roots - metabolism
Plant Shoots - metabolism
Reductase
Reductases
Regulators
Sulfates
Sulfhydryl Compounds - metabolism
Sulfur
Sulfur compounds
Sulfur metabolism
Thiols
Transcription
Up-Regulation
Zinc
Zinc - administration & dosage
Zinc chloride
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Summary:Zinc (Zn) is one of the important elements of plant growth, however, at elevated level it is toxic. Exposure of Chinese cabbage to elevated Zn2+ concentrations (5 and 10 μM ZnCl2) resulted in enhancement of total sulfur and organic sulfur concentration. Transcript level of APS reductase (APR) as a key enzyme in biosynthesis of primary sulfur compounds (cysteine and thiols), was up-regulated in both shoot and root upon exposure to elevated Zn2+, which was accompanied by an increase in the concentration of cysteine in both tissues. In contrast, the concentration of thiols increased only in the root by 5.5 and 15-fold at 5 and 10 μM Zn2+, respectively, which was in accompanied by an upregulation of ATP sulfurylase, an enzyme responsible for activation of sulfate. An elevated content of glucosinolates, mostly indolic glucosinolates, only in the shoot of plants exposed to excess level of Zn2+ coincided with an increase in gene expression of key biosynthetic enzymes and regulators (CYP79B3, CYP83B1, MYB34). Thus distinct acuumulation patterns of sulfur containing compounds in root and shoot of Chinese cabbage may be a strategy for Chinese cabbage to combat with exposure to excess Zn.
ISSN:0176-1617
1618-1328
DOI:10.1016/j.jplph.2019.153088