Hydrogen peroxide is a product of oxygen consumption byTrichomonas vaginalis

The amitochondriate sexually-transmitted human parasitic protozoanTrichomonas vaginalis (Bushby strain) grown anaerobically on complex medium containing cysteine and ascorbic acid consumed O^sub 2^ avidly (6.9 μM min^sup -1^ per 10^sup 6^ organisms) with an apparentK ^sub m^ value of 5.1 μM O^sub 2^...

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Bibliographic Details
Published in:Journal of biosciences 1999-09, Vol.24 (3), p.339-344
Main Authors: Chapman, Alan, Linstead, David J., Lloyd, David
Format: Article
Language:English
Subjects:
Hydrogen peroxide
Oxidative stress
Oxygen
Oxygen consumption
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Summary:The amitochondriate sexually-transmitted human parasitic protozoanTrichomonas vaginalis (Bushby strain) grown anaerobically on complex medium containing cysteine and ascorbic acid consumed O^sub 2^ avidly (6.9 μM min^sup -1^ per 10^sup 6^ organisms) with an apparentK ^sub m^ value of 5.1 μM O^sub 2^ : O^sub 2^ uptake was inhibited by O^sub 2^ > 120 μM. Spectrophotometric assays in the presence of microperoxidase (419-407 nm) indicated that H^sub 2^O^sub 2^ was produced and that inhibition by high O^sub 2^ concentrations was again evident. Hydrogenosomes oxidizing pyruvate in the presence of ADP and succinate showed similar patterns of O^sub 2^ consumption, H^sub 2^O^sub 2^ production (33.5 pmol min^sup -1^ per mg protein), and O^sub 2^ inhibition. Cytosolic NADH oxidase gave no detectable H^sub 2^O^sub 2^, whereas the cytosolic NADPH oxidase produced H^sub 2^O^sub 2^ at a rate (43 pmol min^sup -1^ per mg protein) greater than that of hydrogenosomes. These results are discussed in relation to the oxidative stress experienced by the pathogen in its natural habitat.[PUBLICATION ABSTRACT]
ISSN:0250-5991
0973-7138
DOI:10.1007/BF02941248