Frequency-dependent changes in mitochondrial number and generation of reactive oxygen species in a rat model of vibration-induced injury

• Published in: Journal of Toxicology and Environmental Health, Part A Part A, 2020-01, Vol.83 (1), p.20-35
• Main Author: Krajnak, Kristine
• Format: Article
• Language: English
• Subjects: Adaptive systems; Animals; Apoptosis; Arteries; Arteries - physiopathology; Arterioles; biomarkers; Blanching; Exposure; Frequency dependence; Hand tools; Hand-arm vibration; Innervation; Male; Manual dexterity; Mitochondria; Mitochondria - physiology; Nerves; neuropathy; Occupational exposure; Oxidative Stress; peripheral nerve injury; Rats; Rats, Sprague-Dawley; Reactive oxygen species; Reactive Oxygen Species - metabolism; Receptors; Reduction; Regeneration; Restraint, Physical; Rodents; Tail - blood supply; Tail - pathology; vascular dysfunction; Vascular system; Vasoconstriction; Vibration; Vibration - adverse effects
• ISSN: 1528-7394; 1087-2620; 2381-3504
• DOI: 10.1080/15287394.2020.1718043

Regular use of vibrating hand tools results in cold-induced vasoconstriction, finger blanching, and a reduction in tactile sensitivity and manual dexterity. Depending upon the length and frequency, vibration induces regeneration, or dysfunction and apoptosis, inflammation and an increase in reactive oxygen species (ROS) levels. These changes may be associated with mitochondria, this study examined the effects of vibration on total and functional mitochondria number. Male rats were exposed to restraint or tail vibration at 62.5, 125, or 250 Hz. The frequency-dependent effects of vibration on mitochondrial number and generation of oxidative stress were examined. After 10 days of exposure at 125 Hz, ventral tail arteries (VTA) were constricted and there was an increase in mitochondrial number and intensity of ROS staining. In the skin, the influence of vibration on arterioles displayed a similar but insignificant response in VTA. There was also a reduction in the number of small nerves with exposure to vibration at 250 Hz, and a reduction in mitochondrial number in nerves in restrained and all vibrated conditions. There was a significant rise in the size of the sensory receptors with vibration at 125 Hz, and an elevation in ROS levels. Based upon these results, mitochondria number and activity are affected by vibration, especially at frequencies at or near resonance. The influence of vibration on the vascular system may either be adaptive or maladaptive. However, the effects on cutaneous nerves might be a precursor to loss of innervation and sensory function noted in workers exposed to vibration.