Prevalence and Molecular Subtyping of Blastocystis from Patients with Irritable Bowel Syndrome, Inflammatory Bowel Disease and Chronic Urticaria in Iran

• Published in: Acta parasitologica 2020-03, Vol.65 (1), p.90-96
• Main Authors: Shirvani, Giti, Fasihi-Harandi, Majid, Raiesi, Omid, Bazargan, Nasrin, Zahedi, Mohammad Javad, Sharifi, Iraj, Kalantari-Khandani, Behjat, Nooshadokht, Maryam, Shabandoust, Hajar, Mohammadi, Mohammad Ali, Ebrahimipour, Mohammad, Babaei, Zahra
• Format: Article
• Language: English
• Subjects: Animal Systematics/Taxonomy/Biogeography; Biomedical and Life Sciences; Biomedicine; Blastocystis; Blastocystis - classification; Blastocystis - genetics; Blastocystis Infections - epidemiology; Blastocystis Infections - parasitology; Chi-square test; Chronic Urticaria - epidemiology; Chronic Urticaria - parasitology; Diarrhea; Ecology; Environmental factors; Feces - parasitology; Genetic diversity; Genetic Variation; Genetics; Genotype; Genotypes; Humans; Inflammatory bowel disease; Inflammatory bowel diseases; Inflammatory Bowel Diseases - epidemiology; Inflammatory Bowel Diseases - parasitology; Intestine; Iran - epidemiology; Irritable bowel syndrome; Irritable Bowel Syndrome - epidemiology; Irritable Bowel Syndrome - parasitology; Medical Microbiology; Microbiology; Microbiota; Original Paper; Parasites; Parasitology; Phylogeny; Prevalence; Protozoa; Sequence Analysis, DNA; Statistical tests; Urticaria
• ISSN: 1230-2821; 1896-1851
• DOI: 10.2478/s11686-019-00131-y

Background Blastocystis is a parasite that colonizes in the human intestine. Its clinical features include diarrhea, abdominal pain, or urticarial and irritable bowel syndrome (IBS). Spite of being significant genetic diversity and numerous subtypes within the genus there were no associations between its subtypes and symptomatology. Materials and Methods Aim of this project was subtyping of the protozoa in 184 Iranian people with history of IBS/IBD ( n  = 74) or chronic urticaria ( n  = 59) and individuals referred to general clinic ( n  = 51). Microscopic and molecular examinations used for identifying and subtyping of Blastocystis . Results Overall, frequency of the parasite was 24.46% while, 29.41% of people who referred to general clinic, 20.27%, and 25.42% of IBS/IBD and urticarial cases were infected, respectively. Subtyping result showed that 28.89% of all people were infected with Blastocystis sp. while the prevalence of ST3, ST2 and ST1 were 22.22%, 22.22%, and 17.78%, respectively. Blastocystis sp., was identified in most IBS/IBD cases (46.7%) followed with ST2 and ST3 (13.3 and 13.3, respectively). Whereas, in chronic urticaria group ST2(33.3%) was the major subtype and most individuals in control group were infected with ST3 (33.3%). Pearson’s Chi Square test showed no significant differences between the parasite or subtype prevalence and diseases ( p  > 0.05). Conclusion Given significant factors have effect on clinical signs including host or parasite genetics, microbiota, as well as environmental factors, it seems that further studies are needed to find out different markers of host susceptibility to diverse parasite genotypes in patients with irritable bowel syndrome or urticaria.