Effect of Bone Morphogenic Protein-2-Loaded Mesoporous Strontium Substitution Calcium Silicate/Recycled Fish Gelatin 3D Cell-Laden Scaffold for Bone Tissue Engineering

Bone has a complex hierarchical structure with the capability of self-regeneration. In the case of critical-sized defects, the regeneration capabilities of normal bones are severely impaired, thus causing non-union healing of bones. Therefore, bone tissue engineering has since emerged to solve probl...

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Bibliographic Details
Published in:Processes 2020-04, Vol.8 (4), p.493
Main Authors: Yu, Chun-Ta, Wang, Fu-Ming, Liu, Yen-Ting, Ng, Hooi Yee, Jhong, Yi-Rong, Hung, Chih-Hung, Chen, Yi-Wen
Format: Article
Language:English
Subjects:
3-D printers
Alkaline phosphatase
Biocompatibility
Biological activity
Biomedical materials
Bone biomaterials
Bone healing
Bone morphogenetic protein 2
Bone morphogenetic proteins
Bones
Calcium
Calcium silicates
Cements
Defects
Drug delivery
Drug delivery systems
Engineering research
Gelatin
Growth factors
Hydrogels
Mechanical properties
Nanoparticles
Nonunion
Proteins
Regeneration
Researchers
Scaffolds
Skin & tissue grafts
Strontium
Structural hierarchy
Studies
Tissue engineering
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Summary:Bone has a complex hierarchical structure with the capability of self-regeneration. In the case of critical-sized defects, the regeneration capabilities of normal bones are severely impaired, thus causing non-union healing of bones. Therefore, bone tissue engineering has since emerged to solve problems relating to critical-sized bone defects. Amongst the many biomaterials available on the market, calcium silicate-based (CS) cements have garnered huge interest due to their versatility and good bioactivity. In the recent decade, scientists have attempted to modify or functionalize CS cement in order to enhance the bioactivity of CS. Reports have been made that the addition of mesoporous nanoparticles onto scaffolds could enhance the bone regenerative capabilities of scaffolds. For this study, the main objective was to reuse gelatin from fish wastes and use it to combine with bone morphogenetic protein (BMP)-2 and Sr-doped CS scaffolds to create a novel BMP-2-loaded, hydrogel-based mesoporous SrCS scaffold (FGSrB) and to evaluate for its composition and mechanical strength. From this study, it was shown that such a novel scaffold could be fabricated without affecting the structural properties of FGSr. In addition, it was proven that FGSrB could be used for drug delivery to allow stable localized drug release. Such modifications were found to enhance cellular proliferation, thus leading to enhanced secretion of alkaline phosphatase and calcium. The above results showed that such a modification could be used as a potential alternative for future bone tissue engineering research.
ISSN:2227-9717
2227-9717
DOI:10.3390/pr8040493