Development and characterization of microsatellites from the sweetpotato weevil, Cylas formicarius elegantulus

To conduct population genetics analyses on sweetpotato weevils (Cylas formicarius elegantulus) collected from three populations in the United States, microsatellite loci were developed from sweetpotato weevil transcriptome sequences obtained from a publicly available database. Nineteen of 27 microsa...

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Bibliographic Details
Published in:Journal of applied entomology (1986) 2020-05, Vol.144 (4), p.335-340
Main Authors: Wadl, Phillip A., Trigiano, Robert N., Boggess, Sarah L., Harris‐Shultz, Karen, Williams, Livy H., McQuate, Grant T.
Format: Article
Language:English
Subjects:
Alleles
Brentidae: Coleoptera
Cluster analysis
Cylas formicarius
Gene expression
Genetic diversity
Genetics
Geographical distribution
Geographical locations
Ipomoea batatas
Loci
Microsatellites
Population (statistical)
Population genetics
Population statistics
simple sequence repeats
Statistical analysis
transcriptomes
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Summary:To conduct population genetics analyses on sweetpotato weevils (Cylas formicarius elegantulus) collected from three populations in the United States, microsatellite loci were developed from sweetpotato weevil transcriptome sequences obtained from a publicly available database. Nineteen of 27 microsatellite loci tested were usable for population analyses. Sweetpotato weevil individuals from Georgia (N = 17), Hawaii (N = 16) and South Carolina (N = 12) were analysed. Here, we present microsatellite primer sequences for 19 loci and population genetics statistics, including diversity, population differences and relatedness. Thirty‐nine alleles were detected in the 45 individuals, and four private alleles were observed in individuals from the Hawaii and South Carolina populations. Observed heterozygosities ranged from 0.00 to 1.00, and expected heterozygosities ranged from 0.00 to 0.44. The mean Shannon's information index ranged from 0.30 to 0.49 for the three populations. Pairwise differences among populations (FST estimates) from individuals ranged from a low of 0.022 between Georgia and Hawaii to a high of 0.036 between Georgia and South Carolina, and cluster analysis (PCA and Structure) indicated two populations independent of geographical location.
ISSN:0931-2048
1439-0418
DOI:10.1111/jen.12738