Bacteriocin production by Staphylococcus epidermidis the normal flora of outer ear: a potential probiotic against outer ear infections

Several studies have reported that some infections are resulted from distortions of microflora, and hence, it was hypothesized that exploiting the defensive ability of normal flora may represent a promising approach to treat bacterial pathogens. This work seeks to bring attention to bacteriocin-prod...

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Bibliographic Details
Published in:Journal of biotech research 2020-01, Vol.11, p.13-22
Main Author: Luti, Khalid Jaber Kadhum
Format: Article
Language:English
Subjects:
Antagonism
Antimicrobial agents
Bacteria
Bacterial infections
Bacteriocins
Clinical isolates
Competition
Competitive behavior
E coli
Ear
Ear diseases
Ecosystems
Flora
Infections
Microflora
Pathogens
Probiotics
Screening
Staphylococcus epidermidis
Streptococcus infections
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Summary:Several studies have reported that some infections are resulted from distortions of microflora, and hence, it was hypothesized that exploiting the defensive ability of normal flora may represent a promising approach to treat bacterial pathogens. This work seeks to bring attention to bacteriocin-producing bacteria that exist in the outer ear in order to exploit them to treat outer ear infections. Twelve bacterial isolates were collected from the outer ear of healthy individuals and subjected to a screening program in two steps: primary screening using agar plug diffusion assay to test the competitive activities against each other and secondary screening using well diffusion method. Staphylococcus epidermidis A3 showed an activity against different ear isolates with approximately 160 arbitrary unites (AU)/ml of bacteriocin activity. Tryptic soya broth was selected as the best medium to obtain maximum production of bacteriocin which showed a bactericidal action. In addition, 12 clinical isolates of common ear infections pathogens were used to test the activity of both S. epidermidis cells and its bacteriocin. Results revealed that bacteriocin was effective against 7 isolates, whereas S. epidermidis cells had an antagonism capability against 9 pathogens reflecting a competitive behavior. Moreover, interspecies interactions between Staphylococcus epidermidis A3 and clinical ear pathogens were investigated in liquid cultures which generally showed that bacteriocin production was increased in the presence of Streptococcus pyogenes and Staphylococcus aureus. The present study confirms previous findings and contributes an evidence on the possibility of using S. epidermidis and/or its bacteriocin to protect and against outer ear infections.
ISSN:1944-3285