Dansyl Chloride as a Derivatizing Agent for the Analysis of Biogenic Amines by CZE-UV

• Published in: Chromatographia 2020-06, Vol.83 (6), p.767-778
• Main Authors: Mantoanelli, Jéssica Oliveira Fernandes, Gonçalves, Luís Moreira, Pereira, Elisabete Alves
• Format: Article
• Language: English
• Subjects: Amines; Analytical Chemistry; Beverages; Chemistry; Chemistry and Materials Science; Chlorides; Chromatography; Electrophoresis; Histamine; Laboratory Medicine; Original; Pharmacy; Phosphoric acid; Proteomics; Quality control; Reagents; Selectivity; Tryptamines; Yogurt
• ISSN: 0009-5893; 1612-1112
• DOI: 10.1007/s10337-020-03896-x

Biogenic amines (BAs) are important compounds that can be used in the quality control of food and beverages. BA analysis is a challenging task that can be made easier by applying a derivatizing agent like dansyl chloride (DNS). The optimized capillary zone electrophoresis (CZE) separation of the DNS-BA derivates (derivates of cadaverine, histamine, putrescine, tryptamine, and tyramine) was performed using benzylamine as an internal standard, a potential of 18 kV, a temperature of 23 °C, a running buffer consisting of phosphoric acid, 120 mmol L −1 , pH 2.5, and an hydrodynamic injection at 25 mBar for 6 s. All calibration curves had r 2 higher than 0.99, and limits of detection (LODs) ranged from 7 to 50 µg L −1 . The developed methodology was tested in cheese and yogurt samples. DNS showed to be an alternative derivatization reagent not only because it produced UV-detectable derivates (214 nm), but also because of its stability, aqueous solubility, high selectivity and sensitivity, reduced impurities, and simple preparation steps. Graphic abstract